BackgroundThe etiology and the molecular mechanisms related to breast carcinogenesis remain poorly understood. Some recent reports have examined the role of Human Papillomavirus (HPV) in this disease. The purpose of this study was to determine the prevalence of HPV in breast cancer.MethodsSixty one fresh frozen breast cancers samples were analyzed. Samples were tested for HPV by PCR, and products were automatically sequenced. Findings were correlated with clinical and pathological characteristics.ResultsThe HPV DNA prevalence in the breast cancer samples was 26% (16/61). Clinical parameters were not statistically associated with HPV presence (p>0.05 χ2 test). Sequence analysis in a subgroup of cases indicates the prevalence of low risk HPV11, followed by high risk HPV16. We found no HPV transcriptional activity.ConclusionThe present study demonstrated for the first time in Argentina the presence of HPV in a proportion of the malignant breast tissues. This finding suggests that HPV may have a biological significance in breast carcinogenesis.

BACKGROUND COVID-19 represents a major global health crisis and has resulted in drastic changes to daily life. While even prior to the start of the pandemic college students had a high prevalence of mental health conditions, this group is even more at risk during the pandemic. OBJECTIVE To investigate the emotional impact of quarantine and transition to remote learning on health science students from two countries, regarding anxiety prevalence, mental wellbeing and coping behaviors during the first month of the COVID-19 outbreak. METHODS A total of 28 students from Tecnologico de Monterrey (TEC) in Guadalajara, Mexico and 19 students from Drake University (Drake) in Iowa, United States participated in the study. All students were studying health sciences, including degree programs such as medicine, pharmacy, and nutrition and wellness. This longitudinal study assessed students’ immediate psychological response during the COVID-19 pandemic by using an anonymous online questionnaire administered in April and again in May 2020. The survey was created to assess anxiety levels, coping mechanisms, and changes in daily habits. Demographics such as gender, age, non-academic working, living arrangements, household size, and number of roommates/co-habitants were also collected. RESULTS The April survey sample included 47 students and the May survey sample included 41 students. At least 50% of respondents were females, 20 to 21 years of age. The April survey results showed that more TEC students rated their food intake as higher than before the pandemic when compared to Drake students (p=0.032). Alcohol consumption reportedly stayed the same for Drake students and was lower for TEC students (p=0.004). The May survey results showed a statistically significant difference only for the alcohol consumption (p<0.001). Sleep time between April and May in TEC students showed a significant statistical difference, with students reporting less sleep time in May (p=0.016). The analysis for the level of anxiety according to the Beck Anxiety Inventory showed no statistically significant differences, neither when comparing between intra (TEC p=0.189 and Drake p=0.879) and inter group survey times (TEC vs Drake in April p=0.919 and TEC vs Drake in May p=0.305). The most common physical symptoms reported by students during both study periods were: feeling hot, nervous, fear of losing control, inability to relax, and fear of the worst happening. CONCLUSIONS There is growing concern surrounding the mental health status and needs of health science students during the pandemic. It is important to monitor changes in students’ mental health in response to the pandemic and to create interventions that target students appropriately.