Thirty-seven kittens from eleven litters have been radiographed and eight others have been completely dissected to provide a record of the times of fusion of the epiphyses of the limb bones. The observations are recorded with reference to individual bones and then to the limbs a t approximately monthly intervals. Attention is drawn to the variable presence of the sesamoid bone in the medial head of the gastrocnemius muscle. A comment is made in the discussion on the sequence of fusion of the ossification centres.

A gas chromatographic (GC) method has been developed for determination of cholesterol in meats. The method involves (a) ethanolic KOH saponification of the sample material, (b) homogeneous-phase toluene extraction of the unsaponifiables, (c) derivatization of cholesterol to its trimethylsilylether, and (d) quantitation by GC-flame ionization detection using 5-α-cholestane as internal standard. This direct saponification method is compared with the current AOAC official method for determination of cholesterol in 20 different meat products. The direct saponification method eliminates the need for initial lipid extraction, thus offering a 30% savings in labor, and requires fewer solvents than the AOAC method. It produced comparable or slightly higher cholesterol results than the AOAC method in all meat samples examined. Precision, determined by assaying a turkey meat sample 16 times over 4 days, was excellent (CV = 1.74%). Average recovery of cholesterol added to meat samples was 99.8%.

Abstract— —The incidence of spondylosis deformans in the English and Swedish cat and the English dog is reported. The regional distribution along the column is given for each group and their differences discussed. SUMMARY The incidence of spondylosis deformans in the English and Swedish cat and in the English dog is reported. There is a general increase with advancing age in the proportion affected and this may account for some of the different frequencies that have been published. The method of examination may also have affected results. The distribution of osteophytes along the vertebral column seems to be similar within a species but different between species. It is suggested that different methods. of movement might influence distribution.

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