Preparation of SNEDDS aims to improve solubility and absorption of furosemide in the body to reduce the dosage and minimize the side effects of drugs. Ternary diagram constructed from composition mixture produced nanoemulsion in the range of 20-40% of capryol-90, 20-40% polysorbate-80 and 40-60% PEG-400. Formulations of SNEDDS using Design-Expert ® 10 with simplex-lattice-design method in the study was aimed to investigate the effect of SNEDDS each component's proportions towards test responses. Emulsification time, drug content and viscosity were best demonstrated by run-7 with consecutive values of 131.68±2.14 seconds, 99.89±2.68% and 0.87±0.0043 mm 2 /s. The optimum formula was obtained through entering test response parameter data of all thirteen formula. Drug content and emulsification time was 107.0 ± 1.44% and 155.59±1.56 seconds with viscosity value 0.91±0.00 mm 2 /s. From the physical stability studies, SNEDDS formulas were stable and did not show phase separation when exposed to temparature stress testing.

One of the hepatotoxic condition is caused when consuming large amounts of drugs induced liver injury for a long period of time, such as in TB (tuberculosis) treatment [1]. One of the solutions to prevent the occurrence of hepatotoxicity symptoms in TB patients is by consuming temulawak (Curcuma xanthorriza Roxb.) supplements [2]. Temulawak supplement was given in the form of dessert nata de cocolawak to increase patient interest in consuming it because it is served in a better taste. Parameters product quality of food was determined based on SNI (Indonesian National Standard). This research was conducted in order to increase the economics and environment value of nata de coco products as a functional food. Based on the measurement results, the quality of nata de cocolawak products meets most of the SNI parameters for nata de coco products. Nata de cocolawak products have 9.56% of sugar, 2.11% of crude fiber, level of mineral content are 3.63 mg/Kg of iron, 58.25 mg/Kg of calcium, and 25.73 mg/Kg of zinc, level of metal contaminant are 0.005 ppm of Pb, 0.212 ppm of Cu, and negative levels for As. The Total plate count (TPC) on the product to measure microbial contamination is 5.5.102 CFU/g. Curcumin content as an active compound in the product is 1.306mg/g of sample. The treatment group had smaller ALT, AST, and ALP values than the placebo group. But statistically, these values did not differ significantly (p> 0.05).

The hepatoprotective effects of matoa leaves were evaluated by paracetamol-induced injury in rat's hepatocytes. The ethanolic extract of matoa leaves (EEML) at doses of 200, 300, 500 mg/kg, po and silymarin at dose of 100 mg/kg, po were given for seven days. Silymarin was given as the reference drug. Hepatoprotective effect was studied by measuring the level of AST, ALT, ALP and total protein in serum. In vivo, oral treatment with EEML at dose of 500 mg/kg significantly reduced AST, ALT, ALP in serum whereas total protein was not significantly reduce in each groups. These results indicate that the hepatoprotective action of EEML is likely related to its potent antioxidative activity. Neutralizing reactive oxygen species enhancing the activity of original natural hepatic-antioxidant enzymes may be the main mechanisms of EEML against paracetamol-induced injury.

Temulawak (Curcuma xanthorrhiza Roxb.), traditionally known as a medicinal plant which is effective in reducing blood lipid levels. In this study we made a nata de coco product fortified with temulawak juice called nata de cocolawak. The purpose of this study was to determine the effect of supplementation of nata de cocolawak on total serum cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride-cholesterol, and serum pancreatic lipase levels. Respondents in this study were healthy adult women who were divided into 2 groups, there are group 1 consuming nata de coco products and group 2 consuming nata de cocolawak products. Each product was given as much as 100 grams consumed 3 times a day for 30 days. Nata de cocolawak products contain ascorbic acid, catechins, rutin, quercetin, alkaloids, and curcumin. Supplementation of nata de cocolawak products can significantly reduce serum total cholesterol, LDL-cholesterol, and serum pancreatic lipase levels (p <0.05) and significantly increase HDL-cholesterol levels (p < 0.05) compared to baseline data. While it is not significantly (p>0.05) increase triglyceride-cholesterol levels. In this study, we can prove that regular consumption of nata de cocolawak products can affect blood lipid profile.

Objectives Artocarpus is a tropical plant known for its high concentration of secondary metabolites, especially flavonoids and phenolics. This study aims to evaluate the potential use of ethanol extracts obtained from three species of Artocarpus (Artocarpus altilis, Artocarpus champeden, and Artocarpus heterophyllus) as gastroprotective agents. Methods The gastroprotective potential of the extracts at concentrations of 100, 200, and 400 mg/kg BW was studied in an animal model induced by absolute ethanol. The animals were subjected to extract pre-treatment for 14 days. Subsequently, the gastroprotective potential was evaluated based on the area of the lesion, the volume of gastric fluid, gastric pH, total acidity, gastric wall mucus content, and microscopic lesions. Results There was a significant decrease in the lesion area and gastric fluid volume in the animals that received pre-treated extracts of A. altilis, A. Champeden, and A. heterophyllus, as well as a significant increase in the pH and gastric wall mucus secretion when compared to the ethanol group (p<0.05), rather than the omeprazole or the normal group (p>0.05). Both macroscopic and microscopic examinations revealed a significant decrease in necrotic lesions. Conclusion Extracts of A. altilis, A. Champeden, and A. heterophyllus are effective in lowering the risk of peptic ulcers since they have the potential to be used as natural gastroprotective agents.