As biopotential signals are having very small frequency variations tens of Hz and micro to millivolts amplitude in the range. While acquiring biopotential signals from a human body, intrusions occur in the acquisition system. Thus for acquiring noise-free signals, the enhanced Biopotential Acquisition unit having a high gain with an improved common-mode rejection ratio readout front-end is designed. To solve mentioned issues, a Fully Differential Two-Stage OTA is introduced in this paper to achieve high DC gain and high Unity-gain frequency. Operational Transconductance Amplifier operates at ± 0.2 V supply voltage, the bias current of 20µA, and achieves a high gain of 74dB. The design comprises a frequency compensation structure by connecting a capacitor across a high voltage stage and the load capacitor is connected across output by following the relation of CC > 0.22 CL. The proposed high gain stage achieves additional gain with reduced noise of 0.2 mV/\(\sqrt{\text{H}\text{z}}\) with a minimal power consumption of 1.9 nW. The analog readout front-end is executed using 180 nm CMOS Technology and utilizes less power, making it ideal for battery-powered devices in Biopotential Acquisition systems.