A method is presented for the automated determination of urea nitrogen using the Berthelot reaction for measuring the ammonia released by the enzymatic action of urease. The method compares favorably with the automated diacetyl monoxime method and employs less dangerous and less expensive reagents.

This report describes an interlaboratory comparison of enzymatic serum glucose methods as currently applied in several instrumental adaptations. Four spectrophotometric, hexokinase-based methods used with the Du Pont "aca," the Abbott "Bichromatic Analyzer," the Aminco "Rotochem," and the Technicon "AutoAnalyzer II" were compared with glucose oxidase-based methods as used with the Beckman "Glucose Analyzer" and the "AutoAnalyzer II." We analyzed both normal samples and samples that contained potential interfering substances or were otherwise abnormal. Although all methods were satisfactorily precise, methodological bias was noted in several cases, particularly with abnormal specimens. The hexokinase-based methods were generally more variable. The most consistent data were obtained from the two glucose oxidase methods and the Du Pont aca. Results of this study comprises part of the background information required for development of an accurate glucose reference method under the auspices of the AACC Standards Committee.

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