Heavy metals toxicity is a significant problem for ecological, evolutionary, nutritional and environmental reasons. This study was carried out to evaluate the amount of cadmium, zinc and lead absorption in leaf and root of pine, cypress, plantain and ash in Isfahan, Iran, in 2013. For this purpose, three heavy metals (Cd, Pb and Zn) and three sites (heavy traffic, moderate traffic and control) were chosen based on their effects on human health. The results indicated that the highest and lowest lead and cadmium concentrations belonged to heavy traffic site and control site, respectively. Cd in leaf versus Pb in leaf and Cd in root versus Pb in root had the highest correlation coefficient among the traits indicating positive influence of leaf and root on absorbing Cd and Pb from soil, water and air. In all the studied species, the concentration of Pb was higher than that of Cd and Zn. This was certainty due to the vehicle traffic emitting much more lead than cadmium and zinc. In all the studied species, metal concentration in leaves was higher than in roots, which may be due to high concentration of heavy metals in air than in soil. In this study, Pinus eldarica Medw. tree was found to be the best species to monitor polluted sites in Isfahan city.