SUMMARY During cell division, polarized epithelial cells employ mechanisms to preserve cell polarity and tissue integrity. In dividing cells of the mammalian skin, planar cell polarity (PCP) is maintained through the bulk internalization, equal segregation, and polarized recycling of cortical PCP proteins. The dramatic redistribution of PCP proteins coincides precisely with cell cycle progression, but the mechanisms coordinating PCP and mitosis are unknown. Here we identify Plk1 as a master regulator of PCP dynamics during mitosis. Plk1 interacts with core PCP component, Celsr1, via a conserved polo-box domain (PBD) binding motif, localizes to mitotic endosomes and directly phosphorylates Celsr1. Plk1-dependent phosphorylation activates the endocytic motif specifically during mitosis, allowing bulk recruitment of Celsr1 into endosomes. Inhibiting Plk1 activity blocks PCP internalization and perturbs PCP asymmetry. Mimicking dileucine motif phosphorylation is sufficient to drive Celsr1 internalization during interphase. Thus, Plk1-mediated phosphorylation of Celsr1 ensures PCP redistribution is precisely coordinated with mitotic entry.

ObjectiveTo investigate the effects of different molecular weight (MW), wool derived hydrolysed keratins (i.e. peptides) on the physical properties of relaxed textured hair.MethodsVery curly hair of African origin was relaxed using sodium hydroxide‐based treatment. Relaxed hair was treated with different MW peptides derived from keratin protein and an amino acid, L‐Leucine. The low‐MW keratin peptides were 221 Da, the mid‐MW keratin peptides were approximately 2577 Da, and the high‐MW keratin peptides were approximately 75 440 Da. The penetration of these different peptides into relaxed hair was evaluated using a laser scanning micrometre and by fluorescence microscopy. The effect of these compounds on single‐fibre mechanical properties and thermal properties was evaluated using tensile and DSC testing, respectively.ResultsLow‐ and mid‐MW compounds were able to penetrate deep into the hair cortex. High‐MW peptide adsorbed onto the hair surface and possibly slightly penetrated into the outer layers of the fibre surface. Both mid‐ and high‐MW keratin peptides, increased Young’s modulus and reduced hair breakage at 20% and 80% relative humidity. With the exception of mid‐MW peptide, other peptides and amino acid were not able to modify thermal properties of relaxed textured hair.ConclusionsOur data suggest that low‐MW compounds may increase hair volume, and high‐MW peptides may repair damage on freshly relaxed textured hair.

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