Chia seed is expanding on the market due to its characteristics, but there are few studies on its response to residual fertilization of other crops. The objective was to evaluate the vegetative and productive parameters of common bean as a function of the base fertilization increment and to verify the influence of the residue of this fertilization on the development of chia. The experiment was carried out in two stages, Maringá State University, Umuarama Regional Campus, in a randomized block design with 4 replications. The treatments for the first stage were: T1 - doses recommended for beans and T2, T3, T4 and T5, were recommended doses for beans with increments for each treatment. The evaluated variables were: shoot dry matter, number of pods per plant, grains per plant, grains per pod, 1000 grains weight and yield. In the second stage, the experiment was installed in the same place of the previous cultivation. The treatments were: residual bean fertilization, T6 - plus the treatment with the recommendation for chia. The evaluated variables were: macro and micronutrient leaf contents, shoot dry matter, final plant population, 1.000 grains weight, oil content and yield. For beans and chia, soil samples were collected after harvest to evaluate chemical attributes. In common bean, the results were not significant in the evaluated parameters. In soil, the residual effect of beans was significant for P and K, with 27.2 mg dm −3 and 167.70 mg dm −3 , in treatment T5 and chia was 23.1 mg dm −3 and 89.7 mg dm −3 , for treatment T6, respectively. In chia, yield, oil content and P for leaf macro and micronutrient leaf contents were significant. Thus, the vegetative and productive parameters of the common bean were not influenced by the increase in fertilization. The residual effect was higher for P and K, for beans and chia. For chia, influences by residual effect were observed.

Chia (Salvia hispanicaL.) is an annual herbaceous plant considered as natural omega-3 fatty acids source. To quantify this oil in seeds, a time-consuming method involving financial costs with chemical reagents is used. The objective of this work was verifying existence of correlation between the quantification of oil in chia seeds by means of the Soxhlet method with the ash mass of these seeds obtained by calcination in a muffle oven. For this, samples of chia seeds and the oil quantified by Soxhlet were collected. The same samples were calcined in ash. The simple correlation test was performed, obtaining linear regression. To method validation, a completely randomized design experiment was carried out. The treatments were to quantify the oil by the Soxhlet method and calcination in the muffle using the equation obtained. The objective was to verify the existence of correlation between the oil content of chia seeds, obtained by the Soxhlet method with ash content obtained after burning in the muffle and, therefore, the validation of this method of determination. There is a significant positive correlation between the oil content obtained by Soxhlet method and the ash mass of chia seed. By equation Y = -2.5319x + 16.518, it was noted that it is feasible to quantify the oil content

A chia (Salvia hispanica L.) é uma planta anual herbácea considerada como fonte natural de ácidos graxos ômega-3, fibras, proteínas e antioxidantes. No entanto o cultivo no Brasil ainda é recente e as informações e recomendações técnicas existentes são limitadas, principalmente a respeito de adubação e época de semeadura. Portanto, o presente trabalho objetivou avaliar o efeito da aplicação de doses de fósforo na expressão de genes responsáveis pela síntese de lipídios da cultura da chia. Foi conduzido o experimento em na Fazenda de Universidade Estadual de Maringá no Campus Regional de Umuarama. O solo usado no experimento é um Latossolo Vermelho Distrófico típico, com textura arenosa. O delineamento experimental utilizado foi o inteiramente casualizado com quatro e cinco repetições. Os tratamentos foram compostos por quatro doses de P2O5 aplicados na semeadura (0, 40, 80 e 120 kg ha-1). Foi avaliada a expressão dos genes responsáveis pelo metabolismo de lipídios OLE1 e MGAT. Palavras-chave: Salvia hispânica; teor de óleo; adubação fosfatada.   Lipid metabolism gene expression of chia under phosphorus rates   ABSTRACT: Chia (Salvia hispanica L.) is an annual herbaceous plant considered as a natural omega-3 fatty acids, fibers, proteins and antioxidants source. However, cultivation in Brazil is still recent and the existing information and technical recommendations are limited, mainly regarding fertilization. Therefore, the present work aimed the phosphorus rates application effect on the genes expression responsible for chia plants lipid synthesis. The experiment was conducted at farm of Universidade Estadual de Maringá at the Regional Campus of Umuarama, Paraná, Brazil. The experimental design was a completely randomized design with four and five replications. The treatments were composed of four rates of P2O5 at sowing (0, 40, 80 and 120 kg ha-1). The expression of the genes responsible for lipid metabolism OLE1 and MGAT. Key words: Salvia hispanica; oil meaning; phosphate fertilization.