The objective of this study was to investigate the effects of a gel formulation from the association of Plectranthus neochilus and Cnidoscolus quercifolius on tissue repair in cutaneous wounds in rats. A surgical wound was induced in 35 Wistar rats and treated according to group: G1 - commercial phytotherapeutic gel; G2 - Carbopol gel 1%; G3, 4, 5 - gel formulation from Boldo-gambá and Favela (FGBF) at 2.5%, 5%, and 10%, respectively. 1ml of the product was applied topically daily, for 14 days. Macroscopic evaluation of the wound showed inflammation, granulation, and epithelization in all groups. The FGBF 2.5% group showed greater angiogenic potential. There was a significant difference between the surgical area of the wounds treated with FGBF 2.5%, 5%, or 10% compared to the group with the commercial phytotherapeutic gel. On histomorphometry of the skin, there were reepithelization of the epidermis and superficial dermis, longitudinal collagen fibers, fibroblasts, and blood vessels, and in the deeper dermis, fibroblasts, transverse and longitudinal collagen fibers, blood vessels, and inflammatory cells. The 2.5% formulation had the greatest increase in fibroblast proliferation and most intense collagenization on day 14 of treatment.

No abstract

Concern with antimicrobial resistance in animal production systems increases the interest toward integrative therapies. The objective of the present report was to report an integrative approach to a goat undergoing rumenotomy. A goat with ruminal acidosis underwent rumenotomy, corrective rumen suture, moxibustion, and treatment of the surgical wound (TFO) with sugar. In the first twenty days, acupuncture was performed on ST36, indirect moxibustion on Sp6 and CV8, and TFO with antibiotic ointment and sugar. The wound contracted 2cm, there was gradual return of ruminal dynamics, and moderate pain. Acupuncture continued from D21 to D50 on Yin tang, BL13, BL20, BL23, and BL22. Although there was drainage of ruminal fluid, the wound contracted 4cm during this period, pain was absent, and ruminal dynamics normal. During the last thirty days, the acupuncture technique surround the dragon was used and BL13 point needled, with complete closure of the wound. The TFO from D21 was performed solely with sugar. During treatment, there was modulation of the inflammatory response, with formation of granulation tissue and neovascularization. On D84, fibrinogen was 100mg/dL. The authors conclude that the use of sugar, acupuncture, and moxibustion contributed to return of normal ruminal motility, wound contraction, and complete tissue reepithelization.

Avaliou-se o efeito antimicrobiano in vitro dos extratos etanólicos de Plectranthus neochilus e Cnidoscolus quercifolius de forma isolada e associadas. Na 1ª etapa, utilizaram-se os extratos em concentrações 1%, 2,5%, 5% e 10%. Na 2ª etapa, utilizaram-se apenas extratos brutos. Para o teste de sensibilidade dos extratos, utilizaram-se cepas padrão Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. Nas duas etapas, realizaram-se os mesmos testes de sensibilidade. As placas foram semeadas e lidas após 24 horas de incubação em estufa a 37ºC. De acordo com os resultados, conclui-se que não houve atividade bacteriana dos extratos etanólicos estudados isolados, bem como associados diante das cepas padrão de Staphylococcus aureus, Escherichia coli e Pseudomonas aeruginosa nas diferentes metodologias aplicadas quando comparadas com o antibiótico padrão (amicacina).

Avaliaram-se 20 cães de ambos os sexos, variadas raças, idades e naturalmente infectados pela Ehrlichia canis, que apresentaram sinais clínicos sugestivos da doença e positivos ao teste sorológico como método diagnóstico. Coletou-se sangue para realização de hemograma, teste sorológico e bioquímica sérico. Os sinais clínicos observados com maior frequência foram apatia (75%), vômito (50%), secreção ocular (45%) e distúrbio hemorrágico (5%). Os valores para hemácias, hemoglobina e hematócrito foram abaixo dos valores de referência além de ocorrer uma variabilidade elevada nas variáveis bastonetes e eosinófilos, com valores dos desvios padrão superiores às médias correspondentes, e valores razoavelmente elevados no desvio padrão, nas variáveis segmentados, linfócitos e monócitos.  Os valores para ureia, ALT, AST, FA, e Fósforo encontraram-se dentro dos valores de referência. A proteína plasmática total estava acima dos valores de referência em 55% dos animais. Conclui-se que os sinais clínicos e as alterações laboratoriais encontrados nos cães naturalmente infectados pela Ehrlichia canis não foram sinais específicos para diagnóstico da doença, sendo necessário utilizar outros meios como ferramenta diagnóstica.