Neuronal cell death can occur in a tissue or organ, including the brain, which affects memory. The objectives of this study were to determine the dose of bee venom that causes neuronal death and analyse the alteration of mouse behaviour, focusing in particular on spatial memory. Fifteen male mice of Deutsche Denken Yoken (DDY) strain were divided into control and treatment groups. Bee venom was injected six times for two weeks intraperitoneally with 1.88 mg/kg, 3.76 mg/kg, 5.6 mg/kg, and 7.48 mg/kg doses of venom. Brain histology was studied using haematoxylin-eosin stained paraffin embedded 5 μm coronal sections. A Y maze test was used to assay behaviour. Parameters observed were the number of dead neurons and the percentage of mice with altered behaviour. ANOVA showed that the effects of bee venom were significantly different in the case of the neuronal death parameter but were not significantly different in the case of the mice behaviour parameter. Duncan’s Multiple Range Test (DMRT) demonstrated that P4 (7.48 mg/kg) gave the highest effect of bee venom to promote neuronal death.

Sungkai is a plant that is widely found in Indonesia. This plant is often used in traditional medicine so the biotechnology of endophytic fungi is highly needed due to natural resources from plants that have been reduced. This study reported on endophytic fungi found in sungkai leaves and the compound produced. Morphological and molecular identification through phylogenetic tree analysis was carried out to determine the endophytic fungal species found. Potato Dextrose Broth media was used for the cultivation process. Ethyl acetate was used as a solvent for the extraction and the evaporation process used a rotary evaporator. Antioxidant and antibacterial tests were carried out using the DPPH method and paper disc diffusion. Chromatographic techniques were used to isolate the compound and spectroscopic analysis was performed to identify its chemical structure. The results of the morphological and molecular analysis showed Trichoderma asperellum as an endophytic fungus identified. The pure compound obtained from this endophytic fungus was 4-hydroxybenzoic acid. The antioxidant and antibacterial activity showed a strong category (IC50 = 43.88 ????g/mL; MIC 64 ????g/mL). This compound was very likely to be a raw material for new antibiotics and antioxidants through further research with various modifications.

The study of bay leaf extract had been done to know its potential as Culex quinquefasciatus repellent. Bay leaf extract has the secondary metabolite compounds, such as terpenoid that is known as repellent of mosquitoes. The aim of this study was to determine the effect of bay leaf extract on Culex quinquefasciatus. The method used in this study was an experiment with complete randomized design which was consisted of six treatments and four repetitions. The treatment consisted of control (0%), P1 (10%), P2 (20%), P3 (30%), P4 (40%), and P5 (50%) of bay leaf extract. Data were analyzed by using one-way ANOVA. The results of this study showed the lowest mean of C. quinquefasciatus which repelled was 83% and the highest was 95,5%. Analysis of variance indicated that amount of C. quinquefasciatus which avoided bay leaf extract was significantly different. Based on the result, it can be concluded that bay leaf extract is potential as C. quinquefasciatus repellent.

Biolarvicide is the larvicide derived from plants and relatively safe. The objective of this study was to determine the effect of pletekan leaf powder (Ruellia tuberosa), mimosa (Mimosa pudica), papaya (Carica papaya), and guava (Psidium guajava) on Culex quiquefasciatus larval mortality. The method was an experimental with a completely randomized design consisted of 5 treatments (0 gr, 0,25 gr, 0,5 gr, 0,75 gr, and 1 gr) and 5 repetitions for each plant. The data were analyzed by analysis of variance. Based on the results, leaf powder used in the study, namely guava leaves powder (1 gr), mimosa (1 gr), leaves of pletekan (1 gr), and leaves of papaya (1 gr) showed a very significant effect (P < 0,05) on Culex quiquefasciatus larval mortality. It can be concluded that the pletekan, mimosa, papaya, and guava had the potential as biolarvicide of Culex quinquefasciatus.

The importance of observing the behavior of mice in the laboratory is additional information to compare with their behavior in nature and an effort to increase the welfare of mice (animal welfare) through alleviating the pain and suffering associated with scientific procedures. This study aims to study the daily activities of male mice in the laboratory. The method used was ad libitum and focal animal sampling. Based on the ad libitum sampling method, the daily activities of male mice in the laboratory were divided into 9 activities, namely locomotion, grooming, eating, foraging, social, exploration, resting, making nests, and drinking. Based on the focal animal sampling method, the main activity carried out by male mice in the laboratory was locomotion.