The use of shifted-spectra, first-derivative spectroscopy (or edge detection), and fast Fourier transform filtering techniques for fluorescence rejection in Raman spectra is demonstrated. These techniques take advantage of the fact that Raman signals are very narrow in comparison to fluorescence bands in order to discriminate between the two. None of these techniques require modification of existing instrumentation. Fast Fourier transform filtering and deconvolution techniques also provide a means of improving spectral resolution and the signal-to-noise ratio.

The design of a versatile, thin-layer cell to perform in situ spectroelectrochemistry is discussed. The cell is lowvolume, leak-tight, and easily disassembled. It is constructed of chemically resistant materials and can be used to probe the electrochemical reactions of corrosives as well as moisture-and air-sensitive materials. The use of this cell to monitor the electrochemical reactions of oxyhalide electrolytes by in situ infrared spectroelectrochemistry and pyridine by surface-enhanced Raman scattering spectroscopy is demonstrated.

A remote fiber optic fluorometer system which incorporates a dual wavelength UV laser excitation source is described. The system provides increased specificity for detection of multiple fluorophores without sacrificing real time sensing capability. Limitations imposed by UV transmission in fused silica fibers are discussed.