Elemental carbon nanomaterials (ECNMs) are redox active agents that can be exploited to purposely modify the redox balance of cells. Both pro-or antioxidant properties have been reported. However, to the best of our knowledge, there are not comprehensive studies exploring both properties on the same material in view of a potential application in medicine. At the same time, the effect of the bulk structure on the pro/antioxidant properties is poorly known. Here, carbon nanoparticles (CNPs) derived by glucose with definite size and shape have been prepared, and their redox properties evaluated in cell free systems in the dark or following activation with a Near Infrared (NIR) laser beam (945 nm, 1.3 W/cm 2 ). We found that, when irradiated with NIR, CNPs efficiently generate heat and singlet oxygen ( 1 O2), a property that can be exploited for dual photo-thermal (PT)/ photodynamic (PD) therapy in cancer. On the other hand, in the absence of photo-activation, CNPs react with both oxidant (hydroxyl radicals) and antioxidant (glutathione) species. When tested on a murine macrophages cell line (RAW 264.7) CNPs were clearly antioxidant. Furthermore, albeit efficiently internalized, CNPs do not exert cytotoxic effect up to 80 µg/ml and do not exacerbate TNF-α-mediated inflammation. Overall, the results reported herein suggest that CNPs may represent a new class of safe nanomaterials with potential applications in medicine.

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The paper presents the characterization results of a new all-optical applicator for improved tumor laser ablation treatments that features customized irradiation pattern and builtin temperature sensors. The probe exploits a double cladding optical fiber to integrate some Bragg gratings acting as temperature sensing elements in the core, while guiding the high power beam used for the ablation in the inner cladding. The assessment of the probe behavior has been conducted in two steps: first, with an agar gel phantom to characterize the irradiation pattern and to validate the Bragg grating based measurement setup in comparison with a thermographic camera; then, simulating actual treatments using an ex-vivo animal liver.

The paper presents the characterization results of temperature sensors designed to be employed during thermal treatments of tumors, such as in the laser ablation of malignant cells. The developed sensors are based on Fiber Bragg Gratings, a sensing element able to measure the temperature in proximity of the laser beam without significantly modifying the radiation pattern or perturb the temperature at the sensor site. Different sensor embodiments are analyzed and compared in term of linearity and dynamic response; then a preliminary test during an emulated ablation using a phantom is also presented.

The response of a fiber optic sensor [linearly chirped fiber Bragg grating (LCFBG)] to a linear thermal gradient applied on its sensing length (i.e., 1.5 cm) has been investigated. After these bench tests, we assessed their feasibility for temperature monitoring during thermal tumor treatment. In particular, we performed experiments during ex vivo laser ablation (LA) in pig liver and in vivo thermal ablation in animal models (pigs). We investigated the following: (i) the relationship between the full width at half maximum of the LCFBG spectrum and the temperature difference among the extremities of the LCFBG and (ii) the relationship between the mean spectrum wavelength and the mean temperature acting on the LCFBG sensing area. These relationships showed a linear trend during both bench tests and LA in animal models. Thermal sensitivity was significant although different values were found with regards to bench tests and animal experiments. The linear trend and significant sensitivity allow hypothesizing a future use of this kind of sensor to monitor both temperature gradient and mean temperature within a tissue undergoing thermal treatment.