The possibility to shape stimulus-responsive optical polymers, especially hydrogels, by means of laser 3D printing and ablation is fostering a new concept of “smart” micro-devices that can be used for imaging, thermal stimulation, energy transducing and sensing. The composition of these polymeric blends is an essential parameter to tune their properties as actuators and/or sensing platforms and to determine the elasto-mechanical characteristics of the printed hydrogel. In light of the increasing demand for micro-devices for nanomedicine and personalized medicine, interest is growing in the combination of composite and hybrid photo-responsive materials and digital micro-/nano-manufacturing. Existing works have exploited multiphoton laser photo-polymerization to obtain fine 3D microstructures in hydrogels in an additive manufacturing approach or exploited laser ablation of preformed hydrogels to carve 3D cavities. Less often, the two approaches have been combined and active nanomaterials have been embedded in the microstructures. The aim of this review is to give a short overview of the most recent and prominent results in the field of multiphoton laser direct writing of biocompatible hydrogels that embed active nanomaterials not interfering with the writing process and endowing the biocompatible microstructures with physically or chemically activable features such as photothermal activity, chemical swelling and chemical sensing.

Second Harmonic Generation (SHG) microscopy has gained much interest in the histopathology field since it allows label-free imaging of tissues simultaneously providing information on their morphology and on the collagen microarchitecture, thereby highlighting the onset of pathologies and diseases. A wide request of image analysis tools is growing, with the aim to increase the reliability of the analysis of the huge amount of acquired data and to assist pathologists in a user-independent way during their diagnosis. In this light, we exploit here a set of phasor-parameters that, coupled to a 2-dimensional phasor-based approach (μMAPPS, Microscopic Multiparametric Analysis by Phasor projection of Polarization-dependent SHG signal) and a clustering algorithm, allow to automatically recover different collagen microarchitectures in the tissues extracellular matrix. The collagen fibrils microscopic parameters (orientation and anisotropy) are analyzed at a mesoscopic level by quantifying their local spatial heterogeneity in histopathology sections (few mm in size) from two cancer xenografts in mice, in order to maximally discriminate different collagen organizations, allowing in this case to identify the tumor area with respect to the surrounding skin tissue. We show that the “fibril entropy” parameter, which describes the tissue order on a selected spatial scale, is the most effective in enlightening the tumor edges, opening the possibility of their automatic segmentation. Our method, therefore, combined with tissue morphology information, has the potential to become a support to standard histopathology in diseases diagnosis.

Super-resolution image acquisition has turned photo-activated far-infrared thermal imaging into a promising tool for the characterization of biological tissues. By the sub-diffraction localization of sparse temperature increments primed by the sample absorption of modulated focused laser light, the distribution of (endogenous or exogenous) photo-thermal biomarkers can be reconstructed at tunable ∼10−50 μm resolution. We focus here on the theoretical modeling of laser-primed temperature variations and provide the guidelines to convert super-resolved temperature-based images into quantitative maps of the absolute molar concentration of photo-thermal probes. We start from camera-based temperature detection via Stefan–Boltzmann’s law, and elucidate the interplay of the camera point-spread-function and pixelated sensor size with the excitation beam waist in defining the amplitude of the measured temperature variations. This can be accomplished by the numerical solution of the three-dimensional heat equation in the presence of modulated laser illumination on the sample, which is characterized in terms of thermal diffusivity, conductivity, thickness, and concentration of photo-thermal species. We apply our data-analysis protocol to murine B16 melanoma biopsies, where melanin is mapped and quantified in label-free configuration at sub-diffraction 40 µm resolution. Our results, validated by an unsupervised machine-learning analysis of hematoxylin-and-eosin images of the same sections, suggest potential impact of super-resolved thermography in complementing standard histopathological analyses of melanocytic lesions.

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<p>Under the Paris Agreement, countries are encouraged to preserve and enhance existing carbon sinks, especially forests, thereby including the LULUCF (Land Use, Land Use Change and Forestry) sector in international climate mitigation targets. In particular, Europe has set the target to reach climate neutrality, i.e. a balance between anthropogenic emissions by sources and removals by sinks, by 2050. A prerequisite to reach these goals is an accurate and credible estimation of both these large fluxes. However, recent works highlighted the uncertainty related to the quantification of the land sector mitigation potential, one of the most challenging emission sectors. Moreover, the definition of climate mitigation policies often occur at the local level, where details on CO<sub>2</sub> removals from forests and other land uses are traditionally lacking. Indeed, local authorities  (e.g. cities and Regions) can be more effective in the transition to a sustainable economy compared to higher level authorities such as Nations.</p> <p>In this study, we tested a data-driven method based on eddy covariance (EC) data to quantify the current LULUCF role to the regional carbon sink of the Aosta Valley Region (Italy), by the integration of different approaches. Our model is based on eddy covariance measurements of CO<sub>2</sub> fluxes, MODIS NDVI (250m), daily gridded meteorological variables at 100m spatial resolution, and a land cover map at 250m spatial resolution. A Random Forest model was used to up-scale the point eddy covariance data to the Regional level, by testing different sets of drivers (air temperature, VPD, Snow (presence/absence), NDVI, solar radiation,...). Our model was then compared to independent data derived from the National Forest Inventory (NFI), and a process-based model. Preliminary results show that forests and other ecosystems of the Region remove nearly 70% of the total anthropogenic emissions in this area. The discrepancies between the different methods will be discussed by exploring the different advantages and flaws and the spatio-temporal variability of the different approaches. Such an assessment of the local carbon budget and its uncertainties will provide a solid base for Climate-smart management of the territory and thus for reaching the carbon neutrality targets.</p>