Aim: The purpose of present work was to utilize lignocellulosic forest waste for cost-effective production of commercially important enzymes i.e. cellulase and xylanase by employing co-culture of Aspergillus niger F 7 and Fusarium oxysporum F 8 in solid state fermentation (SSF). Material and Methods: Fungal strains i.e. A. niger F 7 and F. oxysporum F 8 isolated from degrading forest litter were used as co-culture and their enzyme biosynthesis on forest waste was noticed. The prominent forest wastes i.e. Toona ciliata, Celtris australis, Cedrus deodara and Pinus roxburghii were procured from local forest and alkali (NaOH) pretreatment was given to these lignocellulosic substrates to simplify the crystalline cellulose present in them for higher yield of enzymes by fungal isolates. Two types of moistening agents i.e. tap water and modified basal salt medium (BSM) were used in the substrate in the ratio of 1:2 to evaluate their effect on enzyme production under SSF.
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