The no observable effect level was greater than six biweekly bilateral 0.50 mg/eye doses or two biweekly bilateral 1.0 mg/eye doses. These pharmacokinetic and safety data support monthly 1 or 2 mg/eye dose regimens in human clinical trials.

In conclusion, the solution conformation and the molecular structure in the solid state of the [3]-catenand 1 are in good agreement. The geometry of the molecular system (ternary structure) is determined by the formation of optimal intramolecular associations between aromatic subunits, the contribution of intermolecular stacking being also significant in the crystal.Acknowledgment. We thank Dr. C. O. Dietrich-Buchecker and Dr. J. P. Kintzinger for fruitful discussions. The financial support of the CNRS is also gratefully acknowledged. Supplementary Material Available: Tables of atomic coordinates, bond distances and angles, anisotropic thermal parameters, relevant distances in one whole molecule, and relevant dihedral angles between mean planes (9 pages); tables of observed and calculated structure factors (15 pages). Ordering information is given on any current masthead page.

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This unit describes procedures for generating long-range restriction maps of genomic DNA and for analysis of large insert clones. The basic protocol details restriction digestion of agarose-embedded DNA, PFGE separation, Southern transfer, and hybridization. Support protocols describe the preparation of high-molecular-weight genomic DNA samples in agarose blocks and in agarose microbeads, respectively. Additional support protocols describe the preparation of DNA size standards from l phage and two yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe. An alternative method of preparing S. cerevisiae size standards using lithium dodecyl sulfate (LiDS) solubilization is provided. The final protocol details the preparation of BAC DNA suitable for digestion, mapping, and sequencing.