The protocol developed by the Diabetic Retinopathy Grading and Disease Management Working Party represents a new consensus upon which national guidelines can be based leading to the introduction of quality-assured screening for people with diabetes.
Three studies were conducted to evaluate titanium dioxide (TiO2) as a digestibility marker for cattle. In Exp. 1, eight steers consumed prairie hay ad libitum with or without dietary supplements. Fecal recovery of TiO2 averaged 93% and was not affected (P = 0.47) by supplement. Digestibilities calculated with reference to TiO2 were not different (P = 0.15) from those based on total fecal collections. In Exp. 2, two steers were limit-fed corn-based diets. Fecal recovery of TiO2 averaged 95% and that of chromic oxide (Cr2O3) averaged 113%. Digestibilities calculated with reference to TiO2 were underestimated (P < 0.01) by 1.1 percentage units relative to those based on total fecal collections, and those calculated with reference to Cr2O3 were overestimated (P < 0.01) by 2.0 percentage units. In Exp. 3, eight steers in a replicated 4 x 4 Latin square consumed corn-based diets ad libitum. Fecal recovery of TiO2 averaged 90%, whereas that of Cr2O3 averaged 98%. Digestibilities calculated with reference to TiO2 were underestimated (P < 0.01) by 1.6 to 4.3 percentage units, whereas those calculated with reference to Cr2O3 were not different (P = 0.31) from those based on total fecal collections. Future research is warranted to determine the usefulness of TiO2 in measuring digestibility in cattle.
Twelve newborn Holstein bull calves were used to evaluate the effects of dietary abrasiveness, determined by a new method, on ruminal development. Calves were blocked by age and body weight and were assigned to one of three different diets. Each diet had the same ingredients but different particle sizes, which resulted in different abrasive values. No differences were detected in molar percentages of volatile fatty acids in ruminal fluid or in plasma concentrations of urea, glucose, or beta-hydroxybutyrate. The pH of ruminal fluid was lower for calves fed the fine and intermediate diets than for those fed the coarse diet. Digesta-free weights of the stomach and stomach compartments were similar among calves fed the three diets, except that omasum weights were heavier for calves fed the fine diet. Length of the ruminal papillae increased as the abrasive value of the diet decreased. Measurements of ruminal tissue layers from the ventral floor of the cranial sac were not different among diets, but the keratin portion represented more of the epithelial layer for calves fed the diet with the lowest abrasive value, thus decreasing the percentage of metabolically active tissue for those calves.
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