Sugarcane (Saccharum officinarum L.) is one of the most important crops in Kenya and has wide range of economic importance. The sugar industry contributes up to 15% to the country's agricultural gross domestic product and an estimated 25% of the population depends on the industry for their livelihood. However, the industry has been facing several challenges including declining yields due to use of poor quality planting materials. There is an increasing pressure to enhance the productivity of sugarcane in order to sustain profitable sugar industries in Kenya, while there are several diseases attacking sugarcane and reducing its quality. Seed multiplication of newly released varieties of sugarcane is one of the major constraints in Kenya as it takes 6-7 years to produce sufficient quantity of improved seed material. In vitro culture offers a practical and fast method for mass propagation of disease-free clonal materials. Successful protocols for shoot tip culture, callus culture, embryo culture, virus free plant production and somatic embryogenesis have already been established. Thus, in vitro technology can be used to enhance productivity of sugarcane in Kenya. Despite several advantages of applying micro-propagation technique in sugarcane such as quick multiplication of newly released varieties, rejuvenation of old deteriorated varieties; production of disease free seed; easy transportation of seed material; elimination of viruses; high cane productivity and sugar yield etc., this technique is not gaining popularity up to the desired extent. There are several constraints like the high cost of production and appearance of some variants in micropropagated population among others. The present article describes the status, challenges and opportunities of in vitro technology for the sugar industry in Kenya. Though, some problems have now been resolved to considerable extents which have been described in this review however, some constraints still require intensive research work to be resolved so that a safe and efficient exploitation of this technique can be ensured in sugarcane seed production programmes for enhanced yields and quality.
The response of soybean varieties J499, SCS-1 and La suprema, with different growth habits, to intercropping conditions when grown in association with maize H614D was evaluated in a study at Bukura Agricultural College (0° 06'' N; 34° 50'' E), Kenya. The soybean was sown either at the same time as maize or two weeks later in pure stands or intercropped with maize either in the same hill or in alternate rows. The trial was laid out in a randomized complete block design with four replications. Data collected included grain yield, 100 seed weight, percentage germination, and analytical purity among others. Among the three soybean varieties J499 had significantly higher percentage analytical purity (92%) than SCS1 (84%) and La suprema (82%). In terms of % germination, J499 had the highest (93.3%), followed by SCS1 (87.5%) and lastly La suprema irrespective of the intercropping pattern and sowing time. Intercropping La suprema two weeks later in maize led to a 30% increase in 100-seed weight. Sowing at the same time as maize led to a significant difference in seed weight among the varieties, with J499 being the highest (15.1 g) and La suprema the lowest (12.7 g). However, no significant difference was observed when they were sown two weeks later. Among the three varieties, J499 had the lowest number of pods per plant and SCVS-1 the highest. Yields (Kg/ha) of soybean sown in pure stands at the same time as maize were significantly higher (509.9 Kg/ha) than that sown two weeks later (280.2 Kg/ha). The difference in yield between soybean sown at the same time as maize and that sown two weeks later in row intercropping was about 400%. For sowing times pure stands yielded significantly higher soybean yields than intercrops. From the findings it can therefore be concluded that the seed of soybean variety J499 grown as an intercrop has suitable quality attributes to be used as seed.
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