Naskah diterima tanggal 10 Agustus 2012 dan disetujui untuk diterbitkan tanggal 27 Oktober 2012 ABSTRAK. Perbanyakan lili umumnya dilakukan secara vegetatif melalui teknik konvensional menggunakan umbi. Kemampuan totipotensi tanaman memungkinkan setiap bagian tanaman dapat dimanfaatkan untuk perbanyakan tanaman, termasuk tangkai sari bunga. Tujuan penelitian ialah mendapatkan protokol perbanyakan lili menggunakan tangkai sari bunga sebagai eksplan. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Kebun Percobaan Balai Penelitian Tanaman Hias Cipanas, dari Bulan Februari sampai dengan Oktober 2011. Tangkai sari diinduksi membentuk kalus pada beberapa media perlakuan yang mengandung TDZ 0,1-0,4 mg/l, kinetin 0,1-0,4 mg/l, dan 2,4-D 0,05 mg/l. Selanjutnya kalus diregenerasikan menjadi planlet. Penelitian menggunakan rancangan acak lengkap dengan 12 perlakuan media induksi kalus dengan tiga ulangan. Parameter yang diamati ialah waktu inisiasi kalus, bobot basah kalus, jumlah umbi yang terbentuk, serta jumlah daun. Hasil penelitian menunjukkan bahwa media M1-K (MS + TDZ 0,1 mg/l + 2,4-D 0,05 mg/l + kinetin 0,1 mg/l) merupakan media terbaik untuk mendapatkan waktu inisiasi kalus lebih awal dibanding media yang lain. Bobot basah kalus tertinggi diperoleh pada media M3-K (MS + TDZ 0,2 mg/l + 2,4-D 0,05 mg/l + kinetin 0,3 mg/l). Jumlah daun dan jumlah umbi mini tidak berbeda nyata pada media perlakuan yang diuji.Katakunci : Induksi kalus; Regenerasi; Lilium; Tangkai sari; TDZ; 2.4-D; MS ABSTRACT. Kurniati, R, Purwito, A, Wattimena, GA, Marwoto, B, and Supenti 2012. Callus Induction, Bulblets, and Plant Regeneration of Lilium cv. Sorbon from Filament. Lilium is usually propagated vegetatively by using bulb. Based on the totipotency ability of every parts of plant, it is possible to regenerate them into plantlets. The objective of the experiment was to find out micropropagation technique of lily using filament as explant. The experiment was conducted at Tissue Culture Laboratory, Experimental Garden of Indonesian Ornamental Plant Research Institute, Cipanas from February to October 2011. The filaments were cut into 0.5 cm and then those cutting filaments were placed on the several in vitro media containing TDZ 0.1-0.4 mg/l, kinetin 0.1-0.4 mg/l, and 2.4-D 0.05 mg/l to form callus. The callus were subsequently regenerated to be plantlets. A completely randomized design with 12 treatments and three replications were used in this study. Parameters observed were callus initiation time, callus fresh weight, total number of bulb and leaves. The results showed that the M1-K medium (MS + TDZ 0.1 mg/l + 2.4-D 0.05 mg/l + kinetin 0.1 mg/l) was the best medium for callus initiation. The highest of fresh callus weight was achieved on M3-K medium (MS + TDZ 0.2 m g/l + 2.4-D 0.05 mg/l + kinetin 0.3 mg/l). The total of leaves and bulblets of plantlets grown on the tested in vitro media were not significantly different.

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Reduction of chemical application for disease control is important to reduce environmental pollution. An environmental friendly alternative was used Glyocompost. Glyocompost is medium with double functions, there were fungicide and fertilizer. The objectives of the study was to find out best composition of glyocompost and bamboo moss in media for propagation anthurium clones. Anthurium clone “CC” was clone from breeding programme as materials. CC was crossing of Anthurium cv. Cromosum x Anthurium cv. Castano. The composition of media were 1: 18, 1:19, 1: 20 and 1:21 as treatment to apply in CC clones. The parameter observation were plant height, total number of leaves, leaves length, leaves width and total number of shoots. The best composition of glyocompost was obtained 1:19 (glyocompost : bamboo) for leaves width and leaves length. The best of total number of shoots were obtained by composition media 1: 18. While, all treatments were not significantly different for leaves length and total number of leaves. Glyocompost was also accelerated blooming in anthurium clones.

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Lily was usually propagated using MS (Murashige and Skoog) medium supplemented with plant growth regulator and hormone. The important concerns in mass propagation and seed production of lily for industries are cheap, easy and low cost medium. This study’s objective was to find out of generic media to substitute MS medium and to decrease the cost of mass propagation of lily in vitro. Three substitutions medium were Vitagrow, Growmore, Gibril, and G-60. Bulbs of lily Arumsari varieties were used as materials. Complete Random Design with a single factor was used in the experimental design. The treatments were type of generic in vitro medium (Vitagrow, Growmore, Gibril, and G-60), consisting of three replication and 20 units per repetition and five bulbs per unit of repetition. The parameter observed was the total number of leaves and roots, length of leaves and roots, and bulb growth percentage. Growmore medium showed a better result than others in total number of leaves (50,67), length of leaves (1,6 cm), length of roots (0,312 cm) and percentage of bulb growth (100%). The highest total number of roots was achieved in G-60 medium.