Objective : To analyze the effect of DSME (Diabetes Self Management Education) on stress level of Diabetes Mellitus patient in area of Cempaka Public Health Center (Puskesmas) Banjarmasin. Method : This study used pre-experimental design with one group pretest-posttest design. The sample used 15 people who live in area of Cempaka Public Health Center Banjarmasin. Data was analyzed using Wilcoxon test with a confidence level of 95%. Results : Result showed a difference stress level in Diabetes Mellitus patients before and after treated by DSME. Therefore it means that there was significant effect of DSME on stress in Diabetes Mellitus patients (p=0,001 < α 0,005). Conclusion : Self-management education affected to stress level of Diabetes Mellitus patients after treated four weeks.