This study aims to analyze the effectiveness (Practicum Worksheet) of the catalase enzyme that has been used in high schools and provide solutions to solve these problems in the form of LKS reconstruction. The method used is descriptive qualitative analysis. Samples were taken from the Biology class XII book published in 2006-2015 using total sampling. The instrument used was the LKS analysis instrument (conceptual, practical and knowledge construction analysis) to determine the relevance of practicum activities with the curriculum and rubrics based on the Vee Diagram. The results of the analysis using the LKS analysis instrument found the following problems: (1) cognitive problems: 70% of the objectives of the LKS were not in accordance with the work steps; and 60% of LKS competencies are not in accordance with KD; (2) practical problems; 100% LKS do not include time allocation; 90% of LKS did not include safety lab procedures and 60% of LKS did not have appropriate work steps (3) knowledge construction problems: 100% of LKS did not contain questions regarding the character of the facts that emerged; and 70% of worksheets did not contain questions of data analysis and interpretation. Whereas in the results of the analysis using the Vee Diagram, there were no LKS that reached the maximum total score of the Vee Diagram rubric (score 18). The conclusion from the analysis can be seen that the worksheets already have conceptual, practical and knowledge construction components but have not obtained the maximum score from the Vee diagram. So that it is necessary to reconstruct the catalase enzyme lab worksheet. Abstrak. Penelitian ini bertujuan untuk menganalisis efektivitas (Lembar Kerja Praktikum) Enzim Katalase yang selama ini digunakan di SMA dan memberikan solusi pemecahan masalah tersebut dalam bentuk rekonstruksi LKS. Metode yang digunakan adalah analisis deskriptif kualitatif dengan sampel 10 LKS mengenai praktikum enzim katalase pada kurikulum 2013 dan KTSP . Sampel diambil dari buku Biologi kelas XII yang diterbitkan pada tahun 2006-2015 dengan menggunakan cara total sampling. Instrumen yang digunakan adalah instrumen analisis LKS (analisis konseptual, praktikal dan konstruksi pengetahuan) untuk mengetahui relevansi kegiatan praktikum dengan kurikulum dan rubrik berdasarkan Diagram Vee (Novak dan Gowin, 2006). Hasil analisis menggunakan instrumen analisis LKS ditemukan masalah sebagai berikut: (1) masalah kognitif: Hasil analisis menggunakan instrumen analisis LKS ditemukan masalah yang paling mendominasi diantaranya: (1) masalah kognitif : 70% tujuan LKS tidak sesuai dengan langkah kerja; dan 50% kompetensi LKS tidak sesuai dengan KD ; (2) masalah praktikal; 100% LKS tidak mencantumkan alokasi waktu; 90% LKS tidak mencantumkan prosedur safety lab dan 70% LKS tidak memiliki langkah kerja yang tepat (3) masalah konstruksi pengetahuan: 100% LKS tidak memuat pertanyaan mengenai karakter fakta yang muncul; dan 70% LKS tidak memuat interpretasi data pada tabel hasil pengamatan. Sedangkan pada hasil analisis dengan menggunakan Diagram Vee ditemukan tidak ada satu pun LKS yang mencapai skor maksimal dari rubrik Diagram Vee (skor 18). Simpulan dari analisis dapat diketahui bahwa LKS sudah memiliki komponen konseptual, praktikal dan konstruksi pengetahuan namun belum memperoleh skor maksimal dari Diagram Vee. Sehingga perlu adanya rekonstruksi LKS praktikum enzim katalase
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