Higher order emulsions are used in a variety of different applications in biomedicine, biological studies, cosmetics, and the food industry. Conventional droplet generation platforms for making higher order emulsions use organic solvents as the continuous phase, which is not biocompatible and as a result, further washing steps are required to remove the toxic continuous phase. Recently, droplet generation based on aqueous two‐phase systems (ATPS) has emerged in the field of droplet microfluidics due to their intrinsic biocompatibility. Here, a platform to generate all‐aqueous double and triple emulsions by introducing pressure‐driven flows inside a microfluidic hybrid device is presented. This system uses a conventional microfluidic flow‐focusing geometry coupled with a coaxial microneedle and a glass capillary embedded in flow‐focusing junctions. The configuration of the hybrid device enables the focusing of two coaxial two‐phase streams, which helps to avoid commonly observed channel‐wetting problems. It is shown that this approach achieves the fabrication of higher‐order emulsions in a poly(dimethylsiloxane)‐based microfluidic device, and controls the structure of the all‐aqueous emulsions. This hybrid microfluidic approach allows for facile higher‐order biocompatible emulsion formation, and it is anticipated that this platform will find utility for generating biocompatible materials for various biotechnological applications.

Quantitative T2* and T1 relaxometry metrics calculated at 7T have high sensitivity to myelin and can be jointly used to identify dirty-appearing white matter (DAWM) and normal-appearing white matter (NAWM) pathology in post-mortem, multiple sclerosis (MS) brain tissue. Relaxometry mapping was performed on fixed, cerebral brain samples from MS patients and healthy donors. T2* and T1 distributions in WM from MS tissue exhibited bimodality and were shifted to higher relaxation time values compared to healthy tissue. Using k-means clustering applied to 2D T2* and T1 MS tissue data, regions of DAWM were detected in periventricular WM for MS tissue samples.