Motivation to become a teacher is important information to be used in preservice teacher education programs. The purpose of this study was to determine the relationship between demographic factors and motivation to choose teachers as career choices in teacher education students. The type of research used in this research is survey. The research subjects were students of the second year at the Department of Biology Education, Universitas Negeri Yogyakarta. The results showed that hometown of students had a relationship with motivation to become a teacher, students from city areas had higher scores. The results of the analysis on each aspect indicate that hometown of students has a relationship with the aspect of job security (Sig = 0.036) and gender has a relationship with the aspect of work with children and adolescents (Sig = 0.042). In addition, gender and hometown of students have a relationship with aspects of enhancing social equality (Sig = 0.015; Sig = 0.021). The conclusion in this study is that the student's hometown has a relationship with their motivation to become a teacher as a career choice, while gender and family background have no relationship.

Purpose: The aim of this study was to obtain a primer design capable of amplifying the PhtD gene from various S. pneumoniae bacterial serotypes as a protein-based pneumonia vaccine candidate that does not depend on bacterial serotypes.Methods: Primary preparation was carried out through a bioinformatics approach using several software followed by testing the use of primers in the amplification process which was carried out in silico.Results: The results of this study were a pair of primers capable of amplifying the PhtD gene from several S. pneumoniae serotypes.Conclusion: The PhtD gene of the 6 serotypes of S. pneumoniae has high similarities, making it suitable for use as a protein-based vaccine candidate. Based on the consensus of the six serotypes, a pair of primers was obtained that were able to amplify 17 S. pneumoniae strains from several serotypes contained in the reference sequence in silico.