Crambe oil and camelina oil were transesterified with oleyl alcohol, the alcohols derived from crambe and camelina oils, n-octanol or isopropanol using Novozym 435 (immobilized lipase B from Candida antarctica), Lipozyme IM (immobilized lipase from Rhizomucor miehei), and papaya (Carica papaya) latex lipase as biocatalysts. The highest conversions to alkyl esters were obtained with Novozym 435 (up to 95%) in most cases, whereas Lipozyme IM and papaya latex lipase gave lower (40 to 50%) conversions. The conversions with long-chain alcohols (oleyl alcohol, crambe alcohols, and camelina alcohols) were higher (40 to 95%) than with mediumchain n-octanol (30 to 85%). Isopropyl esters of crambe oil and camelina oil were obtained with rather low conversions using Novozym 435 (<40%) and Lipozyme IM (about 10%) as biocatalysts, whereas with papaya latex lipase no isopropyl esters were formed. The conversions of crambe oil and camelina oil to oleyl and n-octyl esters using Novozym 435 as biocatalyst were hardly affected by the ratio of the substrates, but with Lipozyme IM the conversions to alkyl esters distinctly increased with an excess of alcohol substrate.Interest in lipase-catalyzed preparation of wax and bio-esters has grown in the last few years because of the possibility to obtain a wide variety of high-quality products under mild reaction conditions utilizing the substrate selectivity of such biocatalysts (1-9). The alcoholysis of triacylglycerols from vegetable oils with fatty alcohols, catalyzed by lipases, leads to a mixture of long-chain and very long chain wax esters with properties potentially suitable for applications in cosmetics and lubricants (10-15).In an accompanying paper the alkali-catalyzed alcoholysis of crambe oil and camelina oil to produce long-chain esters was studied (16). We report here the solvent-free lipase-catalyzed alcoholysis of these oils with long-chain alcohols, such as oleyl alcohol and alcohols derived from crambe oil and camelina oil with the aim to prepare wax esters as substitutes for jojoba oil. Moreover, lipase-catalyzed alcoholysis of crambe oil and camelina oil with n-octanol and isopropanol was carried out with the aim to prepare products similar to "bio esters" for cosmetics (17). Novozym 435 (immobilized unspecific lipase-B from Candida antarctica) (18-20), Lipozyme IM (immobilized sn-1,3-specific lipase from Rhizomucor miehei) (21,22), and papaya (Carica papaya) latex lipase (23-25) were used as biocatalysts. EXPERIMENTAL PROCEDURESMaterials. Novozym 435 and Lipozyme IM were kind gifts from Novo Nordisk Biotechnologie GmbH (Mainz, Germany). Carica papaya latex was purchased from Sigma-Aldrich-Fluka (Deisenhofen, Germany). The crude latex was ground and sieved to grains <0.8 mm.Refined oils from crambe (Crambe abyssinica) seeds and camelina (Camelina sativa) seeds were provided by the Institut für Pflanzenbau der Landesforschungsanstalt für Landwirtschaft und Fischerei Mecklenburg-Vorpommern (Gülzow, Germany). Fatty acid composition of the oils (designated by number of car...