The geographical location, climate, topography and most important human interference has contributed to the characteristic flora of the old campus of University of Jammu. A total of 24 tree species having 153 individuals belonging to 14 families have been recorded. Out of 24, 23 species belong to Angiosperms (22 dicots and 1 monocot) whereas, only 1 species belong to Gymnosperms. Overall, Moraceae was found to be the dominant family. The total growing stock, total biomass, total carbon content within university campus has been assessed to be 215663.99cm3, 107.83kg, 50.68kg respectively. The total CO2 sequestered by trees and net oxygen produced have been estimated to be 185.84kg and 495.65kg, respectively. Thus, the old campus of University of Jammu with lot of built-up area, roads, lawns, parking places, garden, etc. has sequestered considerably good amount of carbon and also produced considerable amount of oxygen as compared to its size, and its potential for sequestration can be enhanced with the help of management practices and plantation of more trees/shrubs within the permissible areas.