Stimulated by a recent Letter to the Editor showing diffusion artifacts in 3,3'-diaminobenzidine (DAB) cytochemistry (10), we investigated the solubility of the polymer produced in vitro upon oxidation of DAB by ferricyanide at different pH varying from 6.8 to 9.7. We found that with this strong and rapid oxidizing agent no water-soluble polymer was produced over this broad pH range.
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