Medical micromotors have the potential to lead to a paradigm shift in future biomedicine, as they may perform active drug delivery, microsurgery, tissue engineering, or assisted fertilization in a minimally invasive manner. However, the translation to clinical treatment is challenging, as many applications of single or few micromotors require real-time tracking and control at high spatiotemporal resolution in deep tissue. Although optical techniques are a popular choice for this task, absorption and strong light scattering lead to a pronounced decrease of the signal-to-noise ratio with increasing penetration depth. Here, a highly reflective micromotor is introduced which reflects more than tenfold the light intensity of simple gold particles and can be precisely navigated by external magnetic fields. A customized optical IR imaging setup and an image correlation technique are implemented to track single micromotors in real-time and label-free underneath phantom and ex vivo mouse skull tissues. As a potential application, the micromotors speed is recorded when moving through different viscous fluids to determine the viscosity of diverse physiological fluids toward remote cardiovascular disease diagnosis. Moreover, the micromotors are loaded with a model drug to demonstrate their cargotransport capability. The proposed reflective micromotor is suitable as theranostic tool for sub-skin or organ-on-a-chip applications.

Coherent fiber bundle (CFB)-based endoscopes enable optical keyhole access in applications such as biophotonics. In conjunction with objective lenses, CFBs allow imaging of intensity patterns. In contrast, digital optical phase conjugation enables lensless holographic endoscopes for the generation of pixelation-free arbitrary light patterns. For real-world applications, however, this requires a non-invasive in situ calibration of the complex optical transfer function of the CFB with only single-sided access. We show that after an initial calibration in a forward direction, a differential phase measurement of the back-reflected light allows for tracking and compensating of bending-induced phase distortions. Furthermore, we present a novel in situ calibration procedure based on a programmable guide star, which requires access to only one side of the fiber.

Lensless fiber endoscopes are of great importance for keyhole imaging. Coherent fiber bundles (CFB) can be used in endoscopes as remote phased arrays to capture images. One challenge is to image at high speed while correcting aberrations induced by the CFB. We propose the combination of digital optical phase conjugation, using a spatial light modulator, with fast scanning, for which a 2D galvo scanner and an adaptive lens are employed. We achieve the transmission of laser and image scanning through the CFB. Video-rate imaging at 20 Hz in 2D with subcellular resolution is demonstrated in 3D with 1 Hz. The sub-millimeter-diameter scanning endoscope has a great potential in biomedicine, for manipulation, e.g.,  in optogenetics, as well as in imaging.

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