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The acute and chronic toxicities of hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX) to adaphnid (Ceriodaphnia dubia), a hydra (Hydra littoralis), and a midge (Paratanytarsus parthenogeneticus) were examined. RDX concentrations at the solubility limit of the compound under specific test conditions (17.0 mg RDX/L, Ceriodaphnia dubia at 25°C; 32.3 mg RDX/L, Hydra littoralis at 22°C; and 29.2 mg RDX/L, Paratanytarsus parthenogeneticus at 22°C) were not acutely toxic to any of the three tested invertebrates. In a 7‐d survival and reproduction test with Ceriodaphnia dubia, RDX caused no significant effect on survival, but reduced reproductive success. The no‐observed‐effect concentration, lowest‐observed‐effect concentration, and chronic value for Ceriodaphnia dubia were 3.64, 6.01, and 4.68 mg/L, respectively. In an egg‐to‐egg life cycle test with Paratanytarsus parthenogeneticus, survival, growth, egg production, and hatching success were unaffected by RDX. Although not statistically significant, reductions in emergence success were observed at concentrations as low as 6.78 mg/L.

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The acute and chronic toxicities of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) to a daphnid (Ceriodaphnia dubia), a hydra (Hydru littoralis), and a midge (Paratanytarsus parthenogeneticus) were examined. RDX concentrations at the solubility limit of the compound under specific test conditions (17.0 mg RDX/L, Ceriodaphnia dubia at 25°C; 32.3 mg RDX/L, Hydra littoralis at 22°C; and 29.2 mg RDX/L, Paratanytarsusparthenogeneticus at 22°C) were not acutely toxic to any of the three tested invertebrates. In a 7-d survival and reproduction test with Cerioduphnia dubia, RDX caused no significant effect on survival, but reduced reproductive success. The noobserved-effect concentration, lowest-observed-effect concentration, and chronic value for Cerioduphnia dubia were 3.64, 6.01, and 4.68 mg/L, respectively. In an egg-to-egg life cycle test with Paratanytarsus parthenogeneticus, survival, growth, egg production, and hatching success were unaffected by RDX. Although not statistically significant, reductions in emergence success were observed at concentrations as low as 6.78 mg/L.

The acute toxicity of diethyleneglycol dinitrate (DEGDN) to nine freshwater aquatic organisms was determined. Juvenile fathead minnow, bluegill, channel catfish and rainbow trout were exposed to the compound for 96 h. The invertebrates tested for 48 h included the neonate water flea, early-young amphipod, midge larva and the mayfly larva. The effect of DEGDN on the growth of the green alga Selenastrum capricornutum was also studied.The toxicity of DEGDN was relatively low to the nine freshwater species tested. Toxicity values ranged from a 5-d EC50 (standing crop) of less than 58.4 mg/L for the alga to a 96-h LC50 of 491.4 mg/L for the fathead minnow. The most sensitive invertebrate, the daphnid, was more sensitive than the most sensitive fish, the bluegill.