Purpose:To characterize the test-retest variability with supra-aural and insert earphones and compare the pure tone thresholds between the transducers. Methods: Seventy-four individuals of both genders with normal hearing and tympanometric curve of type A were selected. All subjects underwent anamnesis, meatoscopy, air conduction pure tone audiometry, either with the supra-aural earphone, or with insert earphone and speech audiometry. After this initial evaluation Acoustic Impedance Tests were performed. Finally, pure tone audiometry procedures were repeated with both transducers. Results: Supra-aural earphone produced higher test-retest variability when compared to the insert earphones.