Powders obtained from three grape pomaces (Barbera, Chardonnay before distillation, Chardonnay after distillation) were added at two concentration levels (0.8 and 1.6 % w/w) into semi-hard and hard cheeses (Italian Toma-like and Cheddar, respectively) to increase their polyphenol content. Effects on physicochemical characteristics, total phenolic content (TPC), radical scavenging activity (RSA), proteolysis, organic acids content, starter and non-starter bacteria were evaluated during ripening. The amount and the type of powder used did not significantly affect the physicochemical parameters of cheese with the exception of pH their values. Italian Toma-like and Cheddar cheeses fortified with Chardonnay after distillation powder showed at the end of ripening (30 days and 120 days respectively) the highest TPC and RSA values. Proteolysis and microbial counts did not show statistically significant differences between fortified and control cheeses. This study demonstrated that grape pomace powder can be a functional ingredient to increase TPC and RSA in consumers' diets and the addition of this by-product to cheese is an environmentally friendly way to manage winemaking wastes.
Grape skin flours obtained from grape pomace of Chardonnay, Moscato and Pinot noir varieties were used as sources of polyphenolic compounds in yogurt formulation during 3 weeks of storage. Yogurt containing grape skin flour presented significantly higher total phenolic content (+55%), antioxidant activity (+80%) and acidity (+25%) whereas lower pH, syneresis (−10%) and fat (−20%) than control. Procyanidin B1 and vanillic acids were detected only in the yogurt added of Pinot noir flour while gallic acid, catechin and quercitrin were the major phenolic compounds found in the yogurts with Moscato or Chardonnay grape skins. Significant differences were highlighted for acidity and lactose content while total phenolic content, antioxidant activity and lactic acid bacteria trend were stable after production and storage. The liking test performed with consumers showed a loss of textural quality for yogurts fortified with grape skin flours. PRACTICAL APPLICATIONSGrape skin is a nutritious, but underused, by-product of winemaking containing fiber and antioxidants. Using a suitable production design, a new fortified yogurt formulation with grape by-product could be optimized for enhance antioxidant consumers' daily intake. The use of grape skin flour in the development of valueadded food products will be a step toward making new functional foods, and partially solving waste management problem from wine production. The results of this study would provide an opportunity of dairy producer to develop a novel product in agreement with consumers' preferences. This research represents a new approach in the development of novel dairy foods with high nutritional quality and with great potential applications on food industry.
Summary.A sensory-and consumer based approach to optimize cheese enrichment conditions was 3 proposed. Innovative cheeses developed by adding grape skin powders (GSP) from winemaking process to 4 cow's milk curd were described by cheese taster experts and evaluated by consumers for their 5 acceptability. Even though cheese is not an optimal vehicle for GSP enrichment, the adopted approach 6 clearly underlined the effect of the addition of the GSP on the sensory properties of cheese, it pointed out 7 which sensory features were detrimental for the product acceptability and it allowed to obtain information 8 on how to optimize the ingredient use and the process conditions. and with an increased granularity, sourness, saltiness and astringency. The fortification also contributed 38 3 certain vegetable sensations perceived at low intensity (grassy, cereal, nuts), and some potential negative 39 sensations (earthy, animal, winy, varnish). The white color, the homogenous dough, the compact and
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