Pathogenesis of Sclerotinia sclerotiorum (white mould) has been associated with fungal secretion of oxalic acid (OA). Enzymes capable of degrading OA have been utilized to produce transgenic resistant plants. Transgenic soybean lines containing the decarboxylase gene (oxdc) isolated from a Flammulina sp. were produced by the biolistic process. Molecular analysis revealed successful incorporation of the gene into the plant genome and showed that the oxdc gene was transferred to the progeny plants. Sixteen T 2 transgenic events were screened for S. sclerotiorum resistance using a detached leaf assay. The white mould disease progress curve displayed a significant delay in symptom development in all oxdc events compared with the nontransgenic genotype. Area under the disease progress curve (AUDPC) showed reduction in severity ranging from 61% to 96% comparing the oxdc events with the nontransgenic control. One event (OXDC.9.21) presented some plants that did not show any symptoms after 92 h. RT-PCR analysis for detection of oxdc gene transcripts suggested that expression of the oxdc gene is associated with resistance to S. sclerotiorum.

Elephant grass (pennisetum purpureum schum.) has been grown only in cut-and-carry systems for a long time. However, interest in its grazing management has been increasing because of the selection of short-sized genotypes, which have gained new relevance since 1988 with the registration of ‘mott’ grass. in this sense, tall and short-sized genotypes present important differences in morphology, quality and productivity; although, environmental factors also exert influence on these aspects. This review aimed to approach how the elephant grass size can influence the morphophysiological characteristics, management decisions, and exploitation of this species. the morphophysiological aspects and the elephant grass sizes are important to direct their utilization in the different production systems. The research results from previous decades suggest that the particularities of the tall-sized genotypes, such as great forage accumulation, high organic reserves content, and stem elongation, favor their management in cut-and-carry systems and industrial sectors. Conversely, the short-sized elephant grass genotypes present aspects as higher leaf proportion in the harvested forage, leaf/stem ratio, and tillering. these factors facilitate their exploitation in grazing systems.

This two-year study evaluated the effect of Pennisetum purpureum genotypes under rainfed or irrigated conditions, during the dry and rainy seasons, on herbage, leaf, and stem dry matter (DM) accumulation rates, nutritive value, and carbohydrate and protein fractionation. Treatments were tall (Iri 381 and Elefante B) or dwarf (Mott and Taiwan A-146 2.37) genotypes under rainfed or irrigated conditions. Taiwan A-146 2.37 (146 kg DM ha per day) showed similar herbage accumulation rate (HAR) to tall genotypes during the rainy season (124 and 150 kg DM/ha per day, respectively). Dwarf genotypes showed differences in leaf accumulation rate (LAR) (66 and 49 kg DM/ha per day). Mott leaf had less neutral detergent fiber (NDF) (589 g/kg DM) than Taiwan A-146 2.37 (598 g/kg DM), and tall genotypes had generally greater NDF (668 g/kg DM) than the dwarf genotypes. Irrigation increased fiber deposition in the leaf. Stems of all genotypes had lower in vitro digestible dry matter (IVDDM) (378 g/kg DM) under rainfed conditions in the rainy season. Leaf from irrigated plots had 23% more carbohydrate C fraction (160 g/kg CHO) than those from rainfed plots (122 g/kg CHO). Dwarf genotypes had generally greater nutritive value than tall genotypes. These genotypes show promise under irrigation to fill forage gaps during dry periods.

This study investigated whether genotype influences the establishment of Pennisetum purpureum Schumach. The experimental design was a randomized complete blocks with four treatments and eight replications (n=8). The treatments were four genotypes of P. purpureum, two classified as tall sizes: P. purpureum cv. Elephant B and cv. IRI 381; and two as dwarf types: P. purpureum cv. Mott and Taiwan A-146 2.37. They were planted in a tropical wet and dry region of Brazil. Tall genotypes showed superior field sprouting rates (p < 0.05), ranging between 95-99%, while dwarfs varied between 88-90%, however, Elephant B and IRI 381 produced a much lower average number of tillers (31 and 32 linear m-1, respectively), than Taiwan A-146 2.37 and Mott (56 and 41 linear m-1, respectively) (p < 0.05). Dwarf genotypes produced lower biomass yields (p < 0.05), but this was genotype-dependent and did not impact on their establishment. The levels of non-structural carbohydrates (NSC) (>10%) in the planted stems were associated with satisfactory field sprouting of the elephantgrass genotypes. Despite some variations between the genotypes in terms of sprouting, tillering, and growth rates, the kind of genotype had no major significance on the establishment of the elephantgrass.

This study hypothesised that different elephant grass genotypes respond differently in terms of their morphological development to irrigation. The objective of this study was to evaluate how water availability could affect the morphological development of different genotypes of elephant grass. The treatments consisted of four genotypes of elephant grass [Cenchrus purpureus (Schumach.) Morrone], managed under irrigation or not, during a two‐year field trial. The experiment was arranged in a randomised block design in a split‐plot, with four replicates per treatment (n = 4). Between the genotypes, two of them were classified as tall‐sized (IRI 381 and Elephant B), and two as dwarfs (Taiwan A‐146 2.37 and Mott). A three‐way interaction between season, irrigation, and genotype affected the variables plant height, stem diameter, number of dead tillers, light interception (LI), and leaf area index (LAI) (p < .05). The morphological development of all genotypes was negatively impacted by the dry season. All genotypes grew taller during the rainy season (p < .05) and had a greater light interception (62%–80%) compared to the dry period (28%–59%). The genotype Mott, showed comparable LI and LAI to the tall‐sized genotypes, whilst Taiwan A‐146 2.37 was characterized by the lowest values, despite being the genotype with the greatest tiller density (60 tillers m−2) (p < .05). The use of irrigation mitigated the negative effects of the dry season on the plants, however, the type and size (tall vs. dwarf) of the elephant grass genotypes defined the extent of the responses to the use of the irrigation during the dry period.