The objective of the present study was to determine the effects of Roundup on oxidative status in adult Danio rerio liver and gills. Reactive oxygen species (ROS) and antioxidant capacity (ACAP) were measured in fish after exposure to Roundup (5 and 10 mg/L) for 24, 48, 72, and 96 h. Furthermore, gene expression related to antioxidant response was evaluated after 24 and 96 h. In gills, an increase in ACAP was observed after 96 h in the highest concentration. In the liver, a reduction in ROS and ACAP was observed after 24 h, whereas an increase in ACAP was observed after 48 h in the highest concentration. Exposure to the lowest concentration caused a reduction in ROS after 72 and 96 h. Regarding gene expression, a reduction in superoxide dismutase 2 (sod2) and glutathione S-transferase (gstπ) was observed. An increase in uncoupling protein 1 (ucp1) expression was observed in gills of animals exposed to the highest concentration after 24 h. Glutathione peroxidase (gpx) gene expression was reduced in the gills of animals exposed to the lowest concentration; however, it was induced in liver tissue after 96 h of exposure to the highest concentration. These results indicate that zebrafish exposure to Roundup alters oxidative status and causes a response in terms of antioxidant defense system gene expression.

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Fish cellular models are commonly used to study the toxic potential of environmentally relevant compounds. Several of these pollutants act on DNA and compromise its integrity. Little is known, however, about the DNA repair ability of these cellular models. Therefore, the aim of this study was to evaluate the DNA base excision repair (BER) of zebrafish Liver (ZF-L) cell line and primary hepatocytes. We performed kinetic studies of the DNA damage levels after exposure to hydrogen peroxide (HO, 20 μM for 10 min) using the Comet Assay. Ten minutes after HO treatment, 16% and 50% of the initial damage, measured as comet tail length, were repaired in ZF-L cell line and primary hepatocytes, respectively. Primary hepatocytes repaired 50% of the damages twice as fast as ZF-L cell line and showed DNA damage levels similar to control 40 min after HO treatment. The total recovery time for ZF-L model was of 180 min, which indicates the culture cells have a less efficient BER. In conclusion, both ZF-L cell line and primary hepatocytes exhibit BER activity; however, these cellular models have different repair capacity. In addition, we demonstrated that ZF-L cell line and primary hepatocytes are useful tools for ecotoxicological studies focusing on DNA single-strand breaks and BER.