The photoprotective activity of flowers and antibacterial activity of the leaves of Neoglaziovia variegata were investigated. This study also identified the first phenolic compounds of the extracts and fractions of this species by high performance liquid chromatography (HPLC). The photoprotective activity was measured using the spectrophotometric method. The antibacterial activity was evaluated by microdilution method. The chromatographic analysis was performed in HPLC coupled with iode array detector (DAD) detector, using wavelengths of 254 and 320 nm. Nv-Fl-EtOH, Nv-Fl-CHCl 3 and Nv-Fl-AcOEt showed characteristic absorption bands in regions UVB and UVA in a concentration-dependent manner. Nv-Fl-CHCl 3 presented the highest sun protection factor (11.45 ± 2.87). Extracts from the leaves showed activity against most of the microorganisms tested, especially Bacillus cereus, Escherichia coli, Salmonella enterica, Serratia marcescens and Shigella flexneri. HPLC analysis revealed the presence of six phenolics: two flavonoids (isoquercetin and kaempferol-3-O-rhamnoside) and four phenolic acids (caffeic, protocatechuic, p-coumaric and vanillic acids). This result justifies the biological properties, that the species has demonstrated and can lead other phytochemical studies aiming the isolation of these compounds.