This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21.5% of samples, and the predominant coinfection consisted of DENV-1 and DENV-2. Therefore, continuous epidemiological surveillance of DENV in this area is required to predict future forms of dengue heterologous infections and the effect of this on health care.
El objetivo de este trabajo fue determinar la presencia de genes de virulencia en cepas de E. coli aisladas del Río Bravo en la ciudad de Reynosa Tamaulipas. Mediante la técnica de PCR se realizó la detección de los genes que codifican para factores de virulencia como son eae, bfp, stx1 y stx2 en muestras de aguas superficiales del Río Bravo en el municipio de Reynosa Tamaulipas y se identificó que el gen stx2 (27%) fue el más prevalente, seguido de stx1 (10%). Aunque el resto de las muestras fueron consideradas comensales al no tener ninguno de los genes propuestos, la presencia de genes stx1 y stx2 son considerados como un riesgo a la población que tiene contacto con el agua superficial del Río Bravo, por lo cual es necesario un monitoreo microbiológico en esta zona.
Water quality is associated with public health, as it plays an important role in the transmission and epidemiology of pathogens such as Vibrio, since this species have been responsible for human diseases around the world. This study demonstrated the presence of toxigenic Vibrio species in water bodies in Reynosa surroundings, indicating that water bodies may be a source of public health risk.
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