This paper describes the analysis of the propagation of regular and irregular waves in a flume by using Fluent® model, which is based on the Navier-Stokes (NS) equations and employs the finite volume method and the Volume of Fluid (VoF) technique to deal with two-phase flows (air and water). At the end of the flume, a numerical beach is used to suppress wave reflections. The methodology consists of adding a damping sink term to the momentum equation. In this study, this term is calibrated for three cases of regular incident waves (H = 1 m, T = 5, 7.5, and 12 s) by varying the linear and quadratic damping coefficients of the formulation. In general, while lower values of damping coefficients cause residuals on the free surface elevation due to wave interactions with the outlet boundary, reflection occurs on the numerical beach when higher values are used. A range of optimal damping coefficients are found considering one of them null. In one of these cases, temporal series of free surface elevation are compared with theoretical ones and very good agreement is reached. Afterwards, an irregular wave propagation, characterized by a JONSWAP spectrum, is investigated. Several gauges along the flume are evaluated and good agreement between the spectrum obtained numerically and the ones imposed at beginning of the flume is verified. This study shows the capacity of NS models, such as Fluent®, to simulate adequately regular and irregular wave propagations in a flume with numerical beach to avoid reflections.

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The aim of the study was the analysis of Listeria monocytogenes strains in beef samples as well as slaughterhouse environment, located in the Federal District, promote serotyping by polymerase chain reaction (PCR), perform antibiotic susceptibility and submit the strains to Pulsed-field gel electrophoresis (PFGE). A total of 125 beef samples were analyzed, 45 samples of carcasses swabs and 43 swab samples. It detected 13 strains of Listeria monocytogenes, 11 in beef samples. and 2 in slaughterhouse environment. No carcass swabs strains were isolated. Among the 13 strains of L. monocytogenes six strains of serotype 4b were found, five serotype 1/2c and two strains of serotype 1/2a. Among the 11 strains of L. monocytogenes found in beef, one (9.1%) strain showed resistance to erythromycin, one (9.1%) strain to gentamicin, one to ciprofloxacin (9.1%) and all strains (100%) were resistant to nalidixic acid. The two strains coming from the slaughterhouse drains, all (100%) were resistant to nalidixic acid and Sulfonamides. The analysis by pulsed field gel electrophoresis (PFGE) showed 13 different pulsotypes; they were grouped into three different clonal groups, coincidentally correlated with the three different serotypes found, what suggests a widespread dissemination of these profiles in the Federal District, Brazil.