We study the existence of least energy sign-changing solution for the fractional equation (where s ∈ (0, 1) and 2 * s is the fractional critical Sobolev exponent. The proof is based on constrained minimization in a subset of Nehari manifold, containing all the possible sign-changing solutions of the equation. Keywords Fractional Laplacian, sign-changing solutions, critical exponents. 2010 Mathematical Subject Classifications: 49J35, 35S15, and 35B33. = 0, uniformly for a.e. x ∈ Ω. (H3) There exist µ ∈ (2, 2 * s ) such that 0 < µF (x, t) ≤ tf (x, t), for a.e. x ∈ Ω and all t = 0, where F (x, t) := t 0 f (x, τ )dτ .(H4) f (x,t) t is increasing in t > 0 and decreasing in t < 0, for a.e. x ∈ Ω.Remark 1.1 Note that from assumptions (H3) and (H4), we can conclude that the function H(x, t) := tf (x, t) − 2F (x, t) is non-negative, increasing in t > 0 and decreasing in t < 0. Thus, t 2 ∂ t f (x, t) − tf (x, t) = ∂ t H(x, t)t > 0, for a.e. x ∈ Ω and all t = 0.