The aim of this study was to evaluate the accuracy of three-dimensional (3D) reconstructions generated by different software, computed tomography (CT) scanners and slice thicknesses. Ten human dry mandibles were scanned by CT and cone beam CT (CBCT). Digital files were processed in different software systems and 3D reconstructions were performed. Linear measures were made and compared. The results showed significant differences in linear distances between the human dry mandibles and their 3D reconstructions. The relative error from CBCT images ranged from 3.10 to 4.82% and from 3.40 to 5.92% in CT images. It is important to consider that the performance of the software is not just related to the algorithm used, but mostly with its handling, that can facilitate or not the measurement by the operator. In conclusion, the discrepancies were not greater than 0.58 mm, so they should not affect the image quality.
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