One of the major drawbacks of this final disposal technique is leachate production, which occurs due to the degradation of organic matter and rainwater percolation. The leachate composition is quite varied, with high organic load and various compounds that may be toxic to the environment. To reduce the polluting potential of the leachate, it must be subjected to an appropriate treatment. Biological treatments are widely used in the treatment of leachate, although these technologies have some drawbacks. As an alternative to conventional biological treatments, rotating biological reactors are used. The objective of this study was to determine the efficiency of a pilot scale rotating biological reactors in the removal of biochemical oxygen demand, chemical oxygen demand, and phosphorus from the landfill leachate. Effluent toxicity and rotating biological reactors's ability to remove this toxicity were also verified by calculating the germination index of lettuce (Lactuca sativa) and cucumber (Cucumis sativus) seeds. The reactor showed an average efficiency of 75.99% of biochemical oxygen demand removal, 33.53% of chemical oxygen demand removal, and 16.04% of phosphorus removal. In fact, the cucumber and lettuce seeds proved sensitive to the toxicity of the effluent. For crude leachate, the germination index values of lettuce and cucumber were 13.28 and 49.61%, respectively. In this study, rotating biological reactors obtained a good efficiency in the removal of toxicity from leachate. The germination index of the seeds for the treated effluent was 60.9% for lettuce and 82.49% for cucumber.