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This report describes the clinical presentation, pathology and molecular diagnosis of canine parvovirus infection in male Boerboel and female Alsatian puppies. The history of the dogs was considered, examined clinically for vital parameters, haemogram changes and faeces screened for parasites and canine parvovirus faecal antigen. Tissue samples were taken at necropsy for confirmatory diagnosis using histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. There was a severe regenerative anaemia, leucopenia and lymphopaenia. The positive antigen faecal test and pathological findings of haemorrhagic enteritis suggested canine parvoviral enteritis disease. Polymerase chain reaction and sequence analysis confirmed canine parvovirus-2a as the aetiology of the disease. Informed management is important to avoid complications resulting from secondary to severe dehydration, hypovolemia from marked gastrointestinal fluid and protein loss and sepsis from bacterial translocation and leukopenia.

Fowlpox is a relatively slow developing disease characterized by discrete, proliferative lesions on the skin of toes, legs or head, and/or mucous membranes of the mouth and upper respiratory tract of birds. Several diseases of birds such as infectious coryza, infectious laryngotracheitis, Mareks, and vitamin A deficiency can be confused with fowlpox, therefore there is need for an accurate and reliable method of diagnosis. Polymerase Chain Reaction (PCR) and histopathology can be used to arrive at the confirmatory diagnosis of the disease. Consequently, fifty samples from suspected cases of fowlpox from backyard poultry in Ibadan, Nigeria were collected, and PCR was used to detect and characterize the open reading frame 168 (39 kDa core protein) gene of the virus. Also, lesions from the eyelids excised were and histopathology was carried out. Thirty‐one out of 50 samples (62%) tested positive for FPV. Histopathology revealed the presence of dermal hyperplasia and Bollinger bodies which are confirmatory of fowlpox. PCR amplification of 39 kDa core protein gene and histopathology provide a reliable diagnosis of fowlpox.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Confirmatory diagnosis of invasive aspergillosis is paramount to ensure proper treatment and effective management of the disease in food and companion animals. Suspected invasive aspergilllosis in chickens was encountered at post-mortem. Morphological and molecular methods were employed to identify Aspergillus from samples collected from dead chickens at post-mortem. Morphologically, two species of Aspergillus were identified, namely Aspergillus fumigatus and Aspergillus flavus. Molecular identification based on polymerase chain reaction (PCR) and sequence analysis of the partial 5.8 S rRNA, complete internal transcribed spacer-2 and partial 28S rRNA sequences bolstered morphological identification to arrive at the confirmatory diagnosis of the disease. Various hotspots that differentiate A. flavus from A. fumigatus and from other Aspergillus species were identified based on multiple sequence analysis. Maximum likelihood phylogenetic tree showed that isolates from the same species were grouped in the same clade. It is important to correctly identify the Aspergillus species in order to efficiently manage the disease.