Background: Urinary tract infections are one of the major health problem effecting both sexes of all age group. UTIs are often treated with different broad-spectrum antibiotics. The aim of this study was to determine the prevalence of bacteria causing urinary tract infections and their susceptibility pattern from patients reporting in RIMS Hospital.Methods: Mid stream urine (MSU) specimens sent to the laboratory from October 2014 to September 2016 were collected and inoculated onto blood agar and MacConkey agar and incubated at 37ºC for 24 hours. Identification and antibiotic susceptibility test was done following standard operative procedures.Results: 25.66% (1142/4450) samples showed a significant growth out of which 42% (479/1142) were male and 58% (663/1142) were female. E. coli has been found to be the major pathogen causing UTI which account for 61% (696/1142) followed by Staphylococcus aureus 12% (137/1142), CONS 7% (79), Enterococcus spp. 6% (67), Klebsiella spp. 5% (57), Proteus spp. 2% (22), Pseudomonas spp. 2%, Acinetobacter spp. 2% and Candida spp. 3%. Imipenem was the most susceptible antibiotic for Enterobacteriaceae, E. coli (85.9%), Klebsiella spp. (89.4%) and Proteus spp. (95.4%). Vancomycin is 100% sensitive while Linezolid, Nitrofurantoin and Gentamicin are also highly sensitive for both Staphylococcus aureus and CONS.Conclusions: These data may be used to determine trends in antimicrobial susceptibilities, to formulate local antibiotic policies in order to assist clinicians in the rational choice of antibiotic therapy to prevent misuse, or overuse, of antibiotics.

Background: Good hospital hygiene is an integral part of infection control programme. “Microbiological surveillance” provides data about the factors contributing to infection. Bacterial counts in operation theatres are influenced by number of individual present, ventilation and air flow methods. Purpose of the study is to find out prevalence rate of microorganisms in Operation Theatre, to find out the frequency of contamination from various sites in operation theatre.Methods: The study was conducted in the department of microbiology, Regional institute of medical sciences, Imphal, Manipur, India. Air samples were taken by settle plate method in petri dishes containing blood agar and surface samples were taken by a sterile swab soaked in nutrient broth from all operation theatres. The samples were processed according to standard operative procedures.Results: Least bacterial colony forming unit (CFU) was shown by ophthalmology OT 17 CFU/mm3 and highest was shown by emergency OT 200 CFU/mm3. Isolated organism was divided into normal flora (CONS, micrococci), contaminant (bacillus species) and pathogenic organism e.g. Staphylococcus aureus, Acinetobacter spp., Pseudomonas spp. 15 (23.4%) swab samples out of a total of 64 swab samples were found to be growth positive. Out of that 4 CONS, 4 micrococci, 3 Bacillus spp, 2 Acinetobacter spp, 1 Enterobacter spp, 1 Pseudomonas spp. were isolated.Conclusions: Strengthening surveillance and laboratory capacity will surely enhance infection prevention and control. Routine sampling is strongly recommended for increasing awareness to identify and control all possible sources and types of infections.