Background: The outcome of hepatitis B virus (HBV) infection may be influenced by host factors like Human Leukocyte Antigen (HLA).The expression of HLA genes in peripheral blood mononuclear cells (PBMCs) may reflect the molecular mechanism underlying different HBV infection outcomes. Objective: The purpose of the present study was to explore whether HLA DRB1*15 allele confer susceptibility to chronic hepatitis B infected Bangladeshi patients. Method: This cross sectional study was carried out in the Department of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU) during July 2012 to June 2013 for a period of one year.Evaluation of HLA DRB1*15 allele distribution among 30 chronic hepatitis B infected (HBV) Bangladeshi patients compared them with 30 healthy individuals.HLA DRB1*15 allele distribution was detected by conventional PCR followed by agarose gel electrophoresis, using commercial low-resolution DRB1*15 allele polymerase chain reaction sequence specific priming kit. Result: A total of 30 chronic hepatitis B infected (HBV) Bangladeshi patients were evaluated together with 30 healthy controls. The study revealed a significant increase of DRB1*15 allele (46.7% vs 20%; RR= 3.5; X2 = 7.2; P<0.05) compared to healthy controls. This is the first report on HLA DRB1*15 allele associations among chronic hepatitis B (HBV) infected Bangladeshi patients. Conclusion: The present study reveals that HLA DRB1*15 allele was more frequent in chronic hepatitis B infected Bangladeshi patients compared to healthy individuals. Thus, HLA DRB1*15 allele of HLA class II molecules significantly affect the outcome of hepatitis B infection. Bangladesh Journal of Medical Science Vol.18(4) 2019 p.783-788

Background: The course of hepatitis B virus (HBV) infection is not only determined by variations in viral virulence but may be influenced by host immune response, where Human Leukocyte Antigen (HLA) plays an important role. Objective: The purpose of the present study was to explore whether HLA-DRB13* allele of MHC gene had any influence in spontaneous recovery from HBV infection among Bangladeshi adults. Method: This cross sectional study was carried out at the Department of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka Bangladesh. A total of 90 randomly selected hepatitis B virus infected adult patients, consisting of 30 acute HBV infections, 30 chronic HBV infection and 30 healthy controls were selected according to selection criteria for evaluation of HLA DRB1*13 allele. Detection of HLA DRB1*13 allele was done by conventional PCR followed by agarose gel electrophoresis. Result: The study revealed a significant increase of DRB1*13 in acute hepatitis B (AHB vs HC-40% vs 6.7%, RR= 9.4; P value <0.05, AHB vs CHB=40% vs 10%; RR=2.27, P value <0.05) compared to chronic hepatitis B infected (HBV) patients and healthy controls (CHB vs HC-10% vs 6.7%, RR= 1.5, P>0.05). This is the first report on HLA DRB1* gene associations among hepatitis B (HBV) infected Bangladeshi patients. Conclusion: The present study revealed that HLA DRB1*13 was associated with protection against persistent HBV infection among acutely infected adult HBV patients in Bangladesh. Bangladesh Journal of Infectious Diseases 2019;6(2):39-43

Background: Elucidating differences in HLA DRB1* genes distribution may be useful in understanding the molecular pathogenesis of viral hepatitis B.Objective: The aim of the study was to find out the HLA DRB1*15 gene susceptibility among acute and chronic Hepatitis B infected Bangladeshi patients.Methodology: This cross-sectional study was carried out in the Department of Virology at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of June 2012 to June 2013 for a period of one year. Evaluation HLA DRB15*gene distribution was performed among acute hepatitis B and chronic hepatitis B infected (HBV) Bangladeshi patients. HLA DRB15*gene distribution was detected by conventional PCR followed by agarose gel electrophoresis.Result: A total of 60 serologically pre-diagnosed 30 acute hepatitis B and 30 chronic hepatitis B infected (HBV) Bangladeshi patients were evaluated. The study revealed a significant increase of DRB1*15 allele among chronic hepatitis B infected patients compared to acute hepatitis B (46.7% vs 13.3%; RR=5.8, X2 test=7.2; P< 0.05). This is the first report to investigate HLA DRB1* gene associations among acute and chronic HBV infected Bangladeshi patients.Conclusion: In conclusion HLA DRB1*15 is more frequent in chronic hepatitis B infected Bangladeshi patients compared to acute hepatitis B.Bangladesh Journal of Infectious Diseases, June 2018;5(1):3-9