Toxoplasmosis is caused by intracellular parasite Toxoplasma gondii (T. gondii) a zoonotic disease that infects almost all warm-blooded animals, including humans. In addition, T. gondii infection can result in serious and life-threatening consequences for the developing fetus or newborn, including spontaneous abortion, congenital blindness, hydrocephalus, mental retardation, and even death. The aim of this research was to estimate the frequency of seropositivity to IgM and IgG antibodies against T. gondii in pregnant women attended in the health services of the “Lagunera” region of Durango State in the period of January 2018 - October 2018. A cross-sectional study was conducted on 434 pregnant women who used the health services of Durango State. Serum was analyzed for T. gondii infection by detecting IgG and IgM anti-T. gondii antibodies using commercial kits through the immunological technique of ELISA. In addition, a standardized questionnaire was applied to evaluate risk factors and sociodemographic characteristics of the participants. From 434 participants, 4 were detected with IgG antibodies (0.92%) and none of these had a positive result to IgM antibodies (0%). Dry meat consumption (OR = 13.5; 95% CI 0.94 – 192.58; p= 0.055) and other types of meat consumption (OR = 15.61; 95% CI 1.08 – 224.05; p= 0.043) were associated factors to infection. The results showed a lower seroprevalence of antibodies against T. gondii than any other study reported before. In addition, our results indicate the majority of the population studied is at risk of having a primary T. gondii infection.