The aim of the study was to investigate the effects of different levels of rape "00" seeds in the diet on the performance, blood and bone parameters of broilers. A total of 180 1-day-old male Ross 308 chicks were divided into four groups. Group I (the control) was fed on a maize, wheat and soybean diet. Different levels of rape seeds were used in the experimental diets (low, medium and high level in II, III and IV groups, respectively) instead of a soybean meal. The amount of MJ AME N from rape seeds in total AME N was as follows: 0.19-1.51 group II, 0.28-2.26 group III and 0.38-3.01 group IV. The highest level of rape seeds resulted in a lower (P<0.05) body weight gain. The birds from group IV had the lowest feed intake and the poorest (P<0.05) value of FCR at 21 d. The highest level of rape seeds positively influenced (P<0.05) the content of HDL. There were no differences in protein metabolism indices, VLDL, Ca, Pi, bone resistance parameters among dietary treatments at 37 day of age. The results demonstrated that the influence of the increasing level of dietary rape seeds on performance, blood and bone parameters was much more evident in younger birds.