Sociosexuality has been widely studied throughout the world, but there are no psychometric analyses of an instrument to obtain objective measures of this construct in Spanish-speaking America. The Sociosexual Orientation Inventory-Revised (SOI-R) is proposed as a valid and reliable instrument to assess sociosexuality in this region. Exploratory Factorial Analysis (EFA) followed by Confirmatory Factorial Analysis (CFA) was performed to determine the psychometric properties of the SOI-R. Associations between sociosexuality with AIDS phobia and condom use established as measures of external validity. The results show that in a sample of Colombian participants, the three-dimensional structure of the SOI-R is valid and presents high- reliability indices. Likewise, it established that sociosexual behavior, but not attitude and desire, is associated with the frequency of condom use and that sociosexuality has significant associations with AIDS phobia. Finally, we discussed the implications of these findings for Spanish-speaking America.