AbstrakKeikutsertaan pria dalam program KB perlu ditingkatkan dengan menyediakan spermisida alami yang bersumber dari bahan alam yang lebih aman. Salah satunya adalah biji buah dari kalangkala yang merupakan tumbuhan endemik Kalimantan Selatan. Efek spermisida dari ekstrak metanol biji buah kalangkala terhadap spermatozoa mencit dikaji dalam penelitian ini secara in vitro. Sebanyak 25 ekor mencit jantan galur Balb/c berumur 2-3 bulan dengan berat badan 20-30 g digunakan dalam penelitian ini untuk diambil spermatozoanya dengan mengeluarkan sekret cauda epididimis. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan yaitu kontrol dimana suspensi spermatozoa tanpa diberi apapun; Suspensi spermatozoa + NaCMC 0,5%; suspensi spermatozoa + ekstrak biji buah kalangkala 0,1%; suspensi spermatozoa + ekstrak biji buah kalangkala 0,3%; dan suspensi spermatozoa + ekstrak biji buah kalangkala 0.5% dengan 5 ulangan setiap perlakuan. Hasil penelitian ini menunjukkan bahwa ekstrak biji buah kalangkala menyebabkan penurunan motilitas dan kecepatan gerak spermatozoa mencit hingga mencapai nilai nol pada konsentrasi 0,5%. Kata kunci: biji kalangkala, mencit, spermatozoa, spermisida alami Abstract Men involvement in family planning needs to be increased by providing natural spermicide from natural source which is safer. One of them is kalangkala seed which is endemic plant in South
Kalangkala (Litsea angulata) is one of the endemic plants in the wetlands area in Kalimantan which seeds are can reduce the quality of male mice spermatozoa such as motility and mobility. The purpose of this research is to know the potential of kalangkala seed extract on other parameters of spermatozoa quality, which are viability and morphology of male mice spermatozoa in an effort to obtain natural spermicide material. This research used five treatments by using twentyfive male mice. The design of this research was spermatozoa suspensions without being treated (Control = 0), spermatozoa suspensions +Na-CMC 0,5% (P1), spermatozoa suspensions + extract of kalangkala seed 0,1% (P2), spermatozoa suspensions + extract of kalangkala seed 0,3% (P3), and spermatozoa suspensions + extract of kalangkala seed 0,5% (P4), with five repititions for each treatment. The result of this research showed that the kalangkala seed extract reduce the percentage of viability of spermatozoa and increased the percentage of abnormal morphology of spermatozoa male mice. Therefore, kalangkala seed extract has potential as a natural spermicide.
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