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Based on the NPP products of MODIS data, the spatial and temporal distribution characteristics of grassland NPP and its response to climatic factors in the vegetation growing season of the main river valleys in the southern slope of Qilian Mountains from 2000 to 2016 were carried out by correlation analysis and spatial interpolation. The research further provides a scientific basis for the quality evaluation of grassland ecosystems on the southern slope of Qilian Mountain and the rational use of grassland resources along the river. The results show that: (1) With the increasing distance of buffers on both sides of the river, the NPP of grassland in each year shows the characteristics of “single-peak” distribution, which is increased first and then decreased; (2) the NPP of grassland in the main river valley of the southern slope of Qilian Mountain The spatial distribution characteristics show a trend of increasing from northwest to southeast. (3) The spatial distribution of NPP and air temperature in the main river valleys of the southern slope of Qilian Mountains is gradually increasing from northwest to southeast, but the spatial distribution correlation coefficient of NPP and precipitation in the river valley grassland of vegetation growing season basically shows a trendof decreasing from northwest to southeast.

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