Background: Human parechovirus (HPeV) is recognized as a potentially severe viral infection such as gastrointestinal , respiratory and sepsis disease.In neonates and young infants. HPeV-1 is the most prevalent genotype and most commonly causes sepsis in young infants. The aim of study was to determine the prevalence of HPeV in hospitalized young infants with sepsis.Methods: The sera of 100 samples were collected from young infants [46 (46%) females and 54(54%) males < below 90 days] with clinical signs and symptoms of sepsis. The total RNA was extracted, cDNA was synthesized. The Nested PCR was carried out for detection of HPeV. The mean , chi square tests were used for distributions of HPeV genotypes among the gender, age group and season.Results: 5/100 (5%) of patients including 2/46(2%) females and 3/54(3%) males showed positive for HPeV (P=0.85). The results of sequencing and phylogenetic tree revealed that the isolated HPeV were genotype 1.Conclusion: Low prevalence of 5% HPeV were detected. HPeV was dominant in this region. The screening HPeV RNA in patients with sepsis may reduce the use of antibiotics and shorten the duration of hospitalization.