Here, we report trading of endangered shark species in a world hotspot for elasmobranch conservation in Brazil. Data on shark fisheries are scarce in Brazil, although the northern and northeastern regions have the highest indices of shark bycatch. Harvest is made primarily with processed carcasses lacking head and fins, which hampers reliable species identification and law enforcement on illegal catches. We used partial sequences of two mitochondrial genes (COI and/or NADH2) to identify 17 shark species from 427 samples being harvested and marketed on the northern coast of Brazil. Nine species (53%) are listed under some extinction threat category according to Brazilian law and international authorities (IUCN – International Union for Conservation of Nature; CITES – Convention on International Trade of Endangered Species of Wild Fauna and Flora). The number increases to 13 (76%) if we also consider the Near Threatened category. Hammerhead sharks are under threat worldwide, and composed 18.7% of samples, with Sphyrna mokarran being the fourth most common species among samples. As illegal trade of threatened shark species is a worldwide conservation problem, molecular identification of processed meat or specimens lacking diagnostic body parts is a highly effective tool for species identification and law enforcement.

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Carcharhinus limbatus has a cosmopolitan distribution and marked genetic structuring, mainly because of its philopatric behavior. However, analysis of this structuring has not previously included South American populations. In the present study, we analyzed a sample of adult individuals collected on the northern coast of Brazil and compared the sequences of the mitochondrial control region with those of populations already genotyped. Relatively high haplotype diversity (12 haplotypes, genetic diversity of 0.796) was observed, similar to that in other populations but with a much larger number of private alleles. In contrast to populations studied previously, which were represented by neonates, the pronounced allelic variability found in the South American individuals may have resulted from migrations from other populations in the region that have yet to be genotyped. This population was also genetically distinct from the other Atlantic populations (Fst > 0.8), probably because of female philopatry, and apparently separated from the northwestern Atlantic group 1.39 million years ago. These findings indicate that the C. limbatus population from northern Brazil is genetically distinct from all other populations and should be considered as a different management unit for the protection of stocks.

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The cephalopod fauna of the southwestern Atlantic is especially poorly-known because sampling is mostly limited to commercial net-fishing operations that are relatively inefficient at obtaining cephalopods associated with complex benthic substrates. Cephalopods have been identified in the diets of many large marine species but, as few hard structures survive digestion in most cases, the identification of ingested specimens to species level is often impossible. Samples can be identified by molecular techniques like barcoding and for cephalopods, mitochondrial 16S and COI genes have proven to be useful diagnostic markers for this purpose. The Amazon River estuary and continental shelf are known to encompass a range of different substrates with recent mapping highlighting the existence of an extensive reef system, a type of habitat known to support cephalopod diversity. The present study identified samples of the cephalopod fauna of this region obtained from the stomachs of red snappers, Lutjanus purpureus , a large, commercially-important fish harvested by fisheries using traps and hook-and-line gear that are capable of sampling habitats inaccessible to nets. A total of 98 samples were identified using molecular tools, revealing the presence of three squid species and eight MOTUs within the Octopodidae, representing five major clades. These include four known genera, Macrotritopus , Octopus , Scaeurgus and Amphioctopus , and one basal group distinct from all known octopodid genera described here as Lepidoctopus joaquini Haimovici and Sales, new genus and species. Molecular analysis of large predatory fish stomach contents was found to be an incredibly effective extended sampling method for biodiversity surveys where direct sampling is very difficult.