Estudio bioquímico del veneno de picos proteicos (I -VII).Los ensayos de toxicidad han permitido identificar tres toxinas que afectan a Mus musculus y que se encuentran asociadas a los picos IV, V y VII; asimismo, se ha detectado toxicidad sobre Gryllus sp. en los picos IV, V, VI y VII. Entre las actividades enzimáticas ensayadas, se ha encontrado actividad proteolítica sobre caseína en el pico I y actividad de hialuronidasa en el pico IV con una actividad específica de 205.6 μg/min/mg. Tanto en el veneno soluble como en las fracciones colectadas no se encontró actividad de fosfolipasa, anticoagulante ni hemolítica. El trabajo incluye notas sobre la distribución y el hábitat de la especie.Palabras claves: veneno; escorpión; toxina; enzima; Tityus kaderkai. AbstractThe biochemistry of the venom of Tityus kaderkai Kovařik, 2005 from Madre de Dios department, has been studied. The soluble venom contains 47.6% of protein. The venom proteins were separated from 12.9 mg of venom using cationic exchange chromatography in CM Sephadex C-25 with a 0.05 M ammonium acetate buffer pH 7.0. The chromatography profiles show seven peaks of proteins (I -VII) and five protein bands were distinguished in the crude venom, by PAGE-SDS. The toxicity assays allowed the identification of three toxins affecting Mus musculus which were associated to peaks IV, V and VII. Toxic proteins to Gryllus sp. were also found associated to peaks IV, V, VI and VII. Through the enzymatic activity, the presence of proteolytic activity over casein was found related to the first peak. Hyaluronidase activity has also been found in the peak IV with a specific activity 205.6 μg/min/mg. However, the crude venom and collected fractions did not show any phospholipase, anticoagulant, nor hemolytic activity. Notes on the distribution pattern and habitat are also included.