No abstract

Introduction:The robustness of the DNA molecule, coupled with the fact that it contains digital information, makes it ideal as a source of identification which resists weather and environmental aggressions imposed on samples found in crime scenes, but these same factors make these samples exhibiting low DNA concentration and with degradation in your chemical structure. The recovery of remaining genetic material of human skeletons is essential part human forensic identification, because the genetic identification by DNA is a method that outperforms all other existing methods.Objective: This work aims to obtain DNA from bone samples degraded and produce an electropherogram with the minimum number of markers recommended by the scientific community forensic, using a simple and effective protocol. Materials and methods:In order to analyse the DNA, samples of the bones and the teeth was demineralized in an EDTA solution and extracted using the Microcon® tube protocol. PCR analysis system approached by STRs PowerPlex Fusion 6C® system, PowerPlex Fusion® system and Globalfiler® System and Capillary electrophoresis procedures were performed on the ABI 3500 Genetic Analyzer. Results:The DNA extracted from the teeth sample and from the bone sample with demineralization without pulverization presented a male genetic profile complete with all amplification system markers. Conclusions:Our alternative extraction protocol proved to be efficient because it allowed the isolation of DNA from high quality of a 17-year bone buried, in order to present a complete genetic profile of all system markers.