ResumoNo período entre fevereiro de 2006 e novembro de 2007, realizou-se o levantamento florístico de um fragmento de Floresta Ombrófila Mista Aluvial à margem do rio Iguaçu, no município de Palmeira (PR). Espera-se, dessa maneira, contribuir para o conhecimento da flora do Paraná e gerar subsídios para futuros estudos de preservação e recuperação de florestas aluviais. Para tanto, foram alocadas duas parcelas, totalizando aproximadamente 2,5 ha. As coletas de material botânico foram mensais, e, para a determinação das espécies, empregou-se bibliografia específica, eventuais consultas a especialistas e comparações com exsicatas tombadas em herbários da região. A floresta apresentou-se estruturada em três estratos. Coletaram-se indivíduos distribuídos em 67 famílias, 111 gêneros e 134 espécies. As famílias que se destacaram em número de espécies foram Myrtaceae, Asteraceae, Solanaceae, Cyperaceae, Rubiaceae, Bromeliaceae e Poaceae. Muitas das espécies identificadas estavam presentes em outros estudos realizados em ambiente similar, indicando que os resultados são representativos. Palavras-chave: Floresta Ombrófila Mista Aluvial; levantamento florístico; rio Iguaçu. Abstract Floristic survey of one break up of Forest Ombrophylous Mixed Aluvial the Iguaçu river, Palmeira City (PR).During the period from February, 2006 to November 2007, it was developed a survey about a flowering fragment of the Ombrophylous Mixed Aluvial forest in the bank of Iguaçu River in the city of Palmeira (PR). One expects, in this way, to contribute for the knowledge of the Flora of the Paraná and to generate subsidies for future studies of preservation and recovery of aluvial forests. To get to this result it was analyzed two plots that added about 2,5 ha. The collections of botanical materials were monthly, and for the determination of the species specific bibliography it was used, eventual consultations to specialists and comparisons with registered exsiccates in herbaria of the region. The forest was presented structuralized in three stratus. Individuals distributed in 67 families, 111 genera and 134 species had collected themselves. The families which had detached in species number were Myrtaceae, Asteraceae, Solanaceae, Cyperaceae, Rubiaceae, Bromeliaceae and Poaceae. Many of the identified species were found in other studies carried out in similar environment, indicating that the results are representative.

The aim of this study was to compare the influence of the extraction method, chemical composition, antimicrobial effects, antioxidant activity, and cytotoxicity on human cells of the non-polar extracts of grape (Vitis labrusca) and blackberry (Rubus fruticosus) seeds. The Soxhlet (Sox), Bligh–Dyer (BD), and ultrasound (US) methods were used for extractions. For blackberry non-polar seed extract, extraction via the BD method showed the highest mean values of total phenolic content (TPC), expressed in milligrams of gallic acid equivalent per 100 mL of non-polar seed extracts (102.37 mg GAE/100 mL), and higher antioxidant activity in relation to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, expressed in milligrams of gallic acid equivalent per 100 mL of non-polar seed extracts (11.50 mg AAE/100 mL), if compared with the Sox and US extractions. Similar results were obtained for the non-polar grape seed extracts, where BD extraction obtained the highest values for TPC (28.61 mg GAE/100 mL) and DPPH (35.36 mg AAE/100 mL). The type of extraction method had an impact on the composition of fatty acids. Only the non-polar blackberry and grape seed extracts obtained via the Sox method showed some in vitro inhibitory effect against Escherichia coli (IAL 2064) and Staphylococcus aureus (ATCC 13565). Regardless of the extraction method used, the non-polar blackberry and grape seed extracts did not decrease the cell viability (IC50 >1000 µg/mL) of cancer and normal cell lines, thus indicating the relative safety of the extracts. All the seed extracts decreased the generation of reactive oxygen species in the cell lines. Blackberry and grape seed lipid fractions can be utilized as antioxidants, and the extraction methods used cause significant changes in relation to their bioactivity and chemical composition.

The essential oils of the leaves of female (0.13%) and male (0.45%) specimens of Baccharis semiserrata collected in the state of Paraná, Brazil, were analyzed in detail using GC-FID and GC-MS techniques. Both samples have low monoterpene content and present the sesquiterpene spathulenol as their main component, reaching 50.75% in the oil originating from the male specimen and 42.65% in that of the female specimen. Other major sesquiterpenes found were (female-male): aromadendrene (9.61-2.33%), α-muurolene (8.88-2.38%) and δ-cadinene (9.52-3.93%). These data put this plant species forward as potential spathulenol source.

ResumoDurante o estudo taxonômico do gênero Aechmea Ruiz & Pav. no estado do Paraná, Brasil, Aechmea guaratubensis E. Pereira não foi encontrada em campo ou herbários, com exceção do holotypus. Este nome é proposto aqui como nova sinonímia de Aechmea recurvata (Klotzsch) L.B.Sm. Por outro lado, Aechmea triangularis L.B.Sm., conhecida até então como endêmica do estado do Espírito Santo, é registrada pela primeira vez no estado do Paraná. Palavras-chave: Aechmea, distribuição geográfica, taxonomia. AbstractDuring a taxonomic study of the genus Aechmea Ruiz & Pav. in the Paraná State, Brazil, Aechmea guaratubensis E. Pereira was not found in fieldworks or in herbarium collections, unless by the holotypus. This name is proposed here as a new synonym of Aechmea recurvata (Klotzsch) L.B.Sm. Moreover, Aechmea triangularis L.B.Sm., known as endemic from Espírito Santo State, is recorded for the first time in the Paraná State.

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